PMID- 11063799
OWN - NLM
STAT- MEDLINE
DCOM- 20001204
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 121
IP  - 2
DP  - 2000 Sep
TI  - p53 abnormality and chromosomal instability in the same breast tumor cells.
PG  - 150-5
AB  - To clarify the important role of the tumor-suppressor gene p53 in maintaining 
      genetic integrity, we estimated chromosome instability and staining of 
      overexpressed p53 protein in the same cells of five primary breast carcinomas. 
      The method included both fluorescence immunohistochemistry and fluorescence in 
      situ hybridization (FISH) on sections from formalin-fixed, paraffin-embedded 
      breast cancer tissue. By using a centromeric FISH probe for chromosome 17 on 
      interphase cells in these sections, we showed that cells with abnormal p53 
      protein expression had a statistically significant higher number of chromosome 17 
      than did cells with no p53 protein staining in the same samples as well as cells 
      in four other tumor samples with no p53 protein staining. The samples identified 
      positive for p53 abnormality by immunostaining were shown to have p53 mutation by 
      constant denaturing gel electrophoresis analysis and DNA sequencing. These 
      mutated samples were characterized by high DNA index, high S-phase, abnormal 
      karyotype, and aneuploidy. The results strongly implicate p53 mutation as a cause 
      for chromosomal instability and a crucial step in mammary carcinogenesis.
FAU - Sigurdsson, S
AU  - Sigurdsson S
AD  - Molecular and Cell Biology Research Laboratory, Icelandic Cancer Society, 
      Reykjavik, Iceland.
FAU - Bodvarsdottir, S K
AU  - Bodvarsdottir SK
FAU - Anamthawat-Jonsson, K
AU  - Anamthawat-Jonsson K
FAU - Steinarsdottir, M
AU  - Steinarsdottir M
FAU - Jonasson, J G
AU  - Jonasson JG
FAU - Ogmundsdottir, H M
AU  - Ogmundsdottir HM
FAU - Eyfjord, J E
AU  - Eyfjord JE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Breast Neoplasms/*genetics/pathology
MH  - *Chromosome Aberrations
MH  - *Genes, p53
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
EDAT- 2000/11/07 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/07 11:00
PHST- 2000/11/07 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/07 11:00 [entrez]
AID - S0165-4608(00)00260-0 [pii]
AID - 10.1016/s0165-4608(00)00260-0 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2000 Sep;121(2):150-5. doi: 
      10.1016/s0165-4608(00)00260-0.