PMID- 11063817
OWN - NLM
STAT- MEDLINE
DCOM- 20001222
LR  - 20190826
IS  - 0165-5728 (Print)
IS  - 0165-5728 (Linking)
VI  - 111
IP  - 1-2
DP  - 2000 Nov 1
TI  - A role for adrenoceptors in the regulation of pleural neutrophilia induced by 
      LPS.
PG  - 15-22
AB  - The role of catecholamines in regulating pleural neutrophilia evoked by 
      intrathoracic (i.t.) injection of lipopolysaccharide (LPS) was investigated in 
      Wistar rats by means of surgical adrenalectomy, depletion of catecholamine stores 
      or adrenoceptor blockade. Treatment of animals with a single dose of LPS evoked a 
      dramatic increase in the number of pleural neutrophils concomitant with an 
      increase in the number of these cells in blood at 4 h. Although blood 
      neutrophilia was drastically reduced when catecholamine stores were depleted with 
      intraperitoneal (i.p.) injection of reserpine, pleural neutrophilia was not 
      modified. However, the i.t. injection of reserpine reduced the increase in 
      pleural neutrophils after LPS stimulation. Adrenalectomy failed to inhibit the 
      increase in neutrophil counts in the blood or pleural cavity after LPS challenge. 
      Pretreatment with intravenous (i.v.) injection of prazosin, an alpha(1)/alpha(2B) 
      antagonist, reduced LPS-induced blood but not pleural neutrophilia. On the other 
      hand, although pleural neutrophilia was not affected by systemic pretreatment 
      with the alpha(2)-adrenoceptor antagonist, yohimbine, the local treatment (i. t. 
      injection) with this antagonist markedly reduced the increase in pleural 
      neutrophil counts observed after stimulation by LPS. In contrast, pleural 
      neutrophilia induced by i.t injection of formyl-methionyl-leucyl-phenylalanine 
      (fMLP) was not modified by local treatment with yohimbine. Taken together, our 
      results suggest that catecholamines, through activation of alpha(1) and 
      alpha(2)-adrenoceptors, play a role in the regulation of blood and pleural 
      neutrophilia observed during the inflammatory response evoked by LPS in the 
      pleural cavity.
FAU - Altenburg, S P
AU  - Altenburg SP
AD  - Nucleo de Ciencias Comportamentais e do Desenvolvimento, Departamento de 
      Fisiologia e Farmacologia, Instituto Biomedico, Universidade Federal Fluminense 
      (UFF), Rua Ernani Melo 101, S. Domingos, 24210-130, Niteroi, Brazil.
FAU - Paixao e Silva, S
AU  - Paixao e Silva S
FAU - Ventura, D G
AU  - Ventura DG
FAU - Gomes, R N
AU  - Gomes RN
FAU - Bozza, P T
AU  - Bozza PT
FAU - Castro-Faria-Neto, H C
AU  - Castro-Faria-Neto HC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Adrenergic Uptake Inhibitors)
RN  - 0 (Adrenergic alpha-Antagonists)
RN  - 0 (Catecholamines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Receptors, Adrenergic)
RN  - 2Y49VWD90Q (Yohimbine)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 8B1QWR724A (Reserpine)
SB  - IM
MH  - Adrenalectomy
MH  - Adrenergic Uptake Inhibitors/pharmacology
MH  - Adrenergic alpha-Antagonists/pharmacology
MH  - Animals
MH  - Catecholamines/metabolism
MH  - Chemotaxis, Leukocyte/drug effects/immunology
MH  - Lipopolysaccharides/*pharmacology
MH  - Male
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/cytology/*immunology
MH  - Pleura/chemistry/cytology/drug effects/*immunology
MH  - Pleurisy/chemically induced/immunology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, Adrenergic/*immunology/metabolism
MH  - Reserpine/pharmacology
MH  - Yohimbine/pharmacology
EDAT- 2000/11/07 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/07 11:00
PHST- 2000/11/07 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/07 11:00 [entrez]
AID - S0165572800003763 [pii]
AID - 10.1016/s0165-5728(00)00376-3 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2000 Nov 1;111(1-2):15-22. doi: 10.1016/s0165-5728(00)00376-3.