PMID- 11064098
OWN - NLM
STAT- MEDLINE
DCOM- 20010104
LR  - 20190826
IS  - 0165-2478 (Print)
IS  - 0165-2478 (Linking)
VI  - 74
IP  - 3
DP  - 2000 Nov 1
TI  - Systemic or local co-administration of lactoferrin with sensitizing dose of 
      antigen enhances delayed type hypersensitivity in mice.
PG  - 183-8
AB  - Lactoferrin (LF), a major defense protein synthesized and stored in granulocytes 
      has been implicated in maintaining immune homeostasis during an insult-induced 
      metabolic imbalance. In this study, we demonstrated that lactoferrin augments the 
      delayed type hypersensitivity (DTH) response to specific antigens in mice. 
      Lactoferrin (LF) was given to mice orally or intraperitoneally (i.p. ) at the 
      time of immunization, or subcutaneously (s.c.) in a mixture with the immunizing 
      doses of the following antigens, sheep red blood cells (SRBC), Calmette-Guerin 
      bacillus (BCG) or ovalbumin (OVA). A DTH reaction was determined 24 h after 
      administration of an eliciting dose of antigen as a specific increase in foot pad 
      swelling. Lactoferrin enhanced DTH reaction to all studied antigens in a 
      dose-dependent manner. Lactoferrin (LF) given to mice in conjunction with antigen 
      administered in an incomplete Freund's adjuvant induced the DTH response at the 
      level of control mice given antigen in a complete Freund's adjuvant. In addition, 
      LF remarkably increased DTH response to a very small, otherwise non-immunogenic 
      SRBC dose. The increase in DTH response was less pronounced for orally 
      administered LF than for any other routes of administration, however, 
      statistically significant augmentation was demonstrated for each antigen studied. 
      Although the costimulatory action of LF was accompanied by the appearance of 
      bovine lactoferrin-specific cellular responses in mice, it is very unlikely that 
      such responses will be generated in humans, since bovine lactoferrin is a dietary 
      antigen to which a tolerance has been acquired. Considering the involvement of LF 
      in generation of stimulatory signals during the induction phase of an antigen 
      specific immune responses, we suggest that LF may be useful for development of 
      safer and more efficacious vaccination protocols.
FAU - Zimecki, M
AU  - Zimecki M
AD  - Department of Experimental Therapy, Institute of Immunology and Experimental 
      Therapy, Polish Academy of Sciences, Wroclaw, Poland.
FAU - Kruzel, M L
AU  - Kruzel ML
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antigens)
RN  - 0 (BCG Vaccine)
RN  - 9006-59-1 (Ovalbumin)
RN  - EC 3.4.21.- (Lactoferrin)
SB  - IM
MH  - Adjuvants, Immunologic/*administration & dosage
MH  - Administration, Oral
MH  - Animals
MH  - Antigens/*administration & dosage
MH  - BCG Vaccine/administration & dosage/immunology
MH  - Drug Evaluation, Preclinical
MH  - Erythrocytes/immunology
MH  - Female
MH  - Hypersensitivity, Delayed/*etiology/immunology
MH  - Immunity, Cellular
MH  - Immunization/*methods
MH  - Injections, Intraperitoneal
MH  - Injections, Subcutaneous
MH  - Lactoferrin/*administration & dosage
MH  - Male
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Ovalbumin/administration & dosage/immunology
MH  - Sheep
EDAT- 2000/11/07 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/07 11:00
PHST- 2000/11/07 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/07 11:00 [entrez]
AID - S0165247800002601 [pii]
AID - 10.1016/s0165-2478(00)00260-1 [doi]
PST - ppublish
SO  - Immunol Lett. 2000 Nov 1;74(3):183-8. doi: 10.1016/s0165-2478(00)00260-1.