PMID- 11066077
OWN - NLM
STAT- MEDLINE
DCOM- 20010531
LR  - 20190816
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 29
IP  - 4
DP  - 2000 Dec
TI  - Cytogenetic and molecular analysis of the acute monocytic leukemia cell line 
      THP-1 with an MLL-AF9 translocation.
PG  - 333-8
AB  - Cell lines derived from patients with leukemia are used in many molecular biology 
      studies. Here we report the cytogenetic analysis of the THP-1 cell line using 
      G-banding, fluorescence in situ hybridization (FISH), and spectral karyotyping 
      (SKY), and the molecular characterization of the MLL-AF9 rearrangement by RT-PCR. 
      The THP-1 cell line was established from the peripheral blood of a 1-year-old boy 
      with acute monocytic leukemia (AML-M5). THP-1 is near-diploid and consists of two 
      related subclones with a number of aberrations, including the t(9;11), associated 
      with AML M5. The use of FISH allowed us to identify and characterize otherwise 
      hidden cytogenetic rearrangements, which include duplication of the 3' portion of 
      MLL in the derivative 9 chromosome and a deletion of the 5' portion of the AF9 
      gene involved in the translocation. In addition to confirming the FISH results, 
      SKY allowed for a more precise characterization of the karyotype of THP-1 and 
      allowed us to identify other abnormalities in this cell line, including 
      der(1)t(1;12), der(20)t(1;20), deletions 6p, 12p, and 17p, trisomy 8, and 
      monosomy 10. Sequencing of the RT-PCR product showed a direct in-frame fusion 
      product on the derivative chromosome 11 between exon 6 (exon 9) of MLL and exon 5 
      of AF9, which is most commonly involved in MLL-AF9 translocations. This study 
      demonstrates that combining different techniques to achieve a more precise 
      characterization of the THP-1 cell line provides important information that will 
      be valuable for understanding the critical events required for leukemogenesis.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Odero, M D
AU  - Odero MD
AD  - University of Navarra, Pamplona, Navarra, Spain.
FAU - Zeleznik-Le, N J
AU  - Zeleznik-Le NJ
FAU - Chinwalla, V
AU  - Chinwalla V
FAU - Rowley, J D
AU  - Rowley JD
LA  - eng
GR  - CA 42557/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (KMT2A protein, human)
RN  - 0 (MLLT3 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Transcription Factors)
RN  - 149025-06-9 (Myeloid-Lymphoid Leukemia Protein)
RN  - EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Chromosome Aberrations/genetics
MH  - Chromosome Banding
MH  - Chromosome Disorders
MH  - Chromosomes, Human, Pair 9/genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Histone-Lysine N-Methyltransferase
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Monocytic, Acute/*genetics
MH  - Male
MH  - Molecular Sequence Data
MH  - Myeloid-Lymphoid Leukemia Protein
MH  - Nuclear Proteins/*genetics
MH  - Oncogene Proteins, Fusion/genetics
MH  - *Proto-Oncogenes
MH  - *Transcription Factors
MH  - Translocation, Genetic/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2000/11/07 11:00
MHDA- 2001/06/02 10:01
CRDT- 2000/11/07 11:00
PHST- 2000/11/07 11:00 [pubmed]
PHST- 2001/06/02 10:01 [medline]
PHST- 2000/11/07 11:00 [entrez]
AID - 10.1002/1098-2264(2000)9999:9999<::AID-GCC1040>3.0.CO;2-Z [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2000 Dec;29(4):333-8.