PMID- 11068652
OWN - NLM
STAT- MEDLINE
DCOM- 20010301
LR  - 20220316
IS  - 1050-6586 (Print)
IS  - 1050-6586 (Linking)
VI  - 14
IP  - 5
DP  - 2000 Sep-Oct
TI  - Nasal polyposis: from cytokines to growth.
PG  - 279-90
AB  - Nasal polyposis (NP) is a chronic inflammatory condition that is mostly 
      characterized by an infiltration of eosinophils. How this eosinophilic 
      inflammation leads to polyp formation remains largely unclear. In order to 
      identify the most important factors in polyp growth, first we report the 
      histologic features of two early stage manifestations of eosinophilic nasal 
      polyps compared to their surrounding normal mucosa and mature polyps from the 
      same patients. Histomorphologic analysis of these early stage manifestations of 
      NP showed the presence of eosinophils, forming a subepithelial cap over a 
      pseudocyst area that was filled with albumin. In mature NP, a large pseudocyst 
      area containing albumin was surrounded by subepithelial eosinophilia. Second, in 
      an approach to quantify and to study possible relations between eosinophilic 
      inflammation and changes in extracellular tissue components we measured 
      interleukin-5 (IL-5), eotaxin, eosinophil cationic protein (ECP), leukotrienes 
      (LTC4/D4/E4), transforming growth factor-beta 1 (TGF-beta 1), fibronectin, 
      hyaluronic acid, and albumin in nasal tissue homogenates of 31 subjects. Nasal 
      polyp samples (n = 16) were obtained during routine endonasal sinus surgery, 
      whereas control non-polyp samples (n = 15) from subjects with (6) and without (9) 
      allergic rhinitis were obtained from the inferior turbinate during septum 
      surgery. In the group of polyp patients 11 received no treatment, whereas 5 were 
      treated with oral glucocorticoids (GCS) within 4 weeks before surgery. IL-5 was 
      measurable in 8 of 11 untreated NP, whereas IL-5 could not be detected in all 15 
      controls nor in 4 of 5 oral corticoid-treated polyps. The comparison between the 
      untreated polyp group and controls showed significantly higher concentrations of 
      IL-5, eotaxin, ECP, and albumin in polyp supernatants, whereas TGF-beta 1 was 
      significantly lower. In the oral GCS-treated group, ECP and albumin were 
      significantly reduced compared to untreated nasal polyps. The same tendency, but 
      not reaching significance, was seen for eotaxin and fibronectin, while no 
      difference was found for LTC4/D4/E4 and hyaluronic acid between the groups. Our 
      observations suggest a deposition of albumin (and possibly other plasma proteins) 
      and extracellular matrix proteins, which may be regulated by the subepithelial 
      eosinophilic inflammation, as a possible pathogenic principle of polyp formation 
      and growth. IL-5 and eotaxin are found to be key factors for eosinophilic 
      accumulation and activation in NP. Oral corticoid treatment may lead to the 
      shrinkage of NP by downregulation of the eosinophilic inflammation and reduction 
      of the extravasation and deposition of albumin in NP.
FAU - Bachert, C
AU  - Bachert C
AD  - Department of Otorhinolaryngology, Ghent University Hospital, Belgium.
FAU - Gevaert, P
AU  - Gevaert P
FAU - Holtappels, G
AU  - Holtappels G
FAU - Cuvelier, C
AU  - Cuvelier C
FAU - van Cauwenberge, P
AU  - van Cauwenberge P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Rhinol
JT  - American journal of rhinology
JID - 8807268
RN  - 0 (Albumins)
RN  - 0 (Blood Proteins)
RN  - 0 (CCL11 protein, human)
RN  - 0 (Chemokine CCL11)
RN  - 0 (Chemokines, CC)
RN  - 0 (Cytokines)
RN  - 0 (Eosinophil Granule Proteins)
RN  - 0 (Fibronectins)
RN  - 0 (Interleukin-5)
RN  - 0 (Leukotrienes)
RN  - 0 (Transforming Growth Factor beta)
RN  - 9004-61-9 (Hyaluronic Acid)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Albumins/analysis
MH  - Blood Proteins/analysis
MH  - Chemokine CCL11
MH  - *Chemokines, CC
MH  - Cytokines/*analysis
MH  - Eosinophil Granule Proteins
MH  - Eosinophils/pathology
MH  - Fibronectins/analysis
MH  - Humans
MH  - Hyaluronic Acid/analysis
MH  - Immunohistochemistry
MH  - Interleukin-5/analysis
MH  - Leukotrienes/analysis
MH  - Nasal Polyps/drug therapy/*etiology/pathology
MH  - *Ribonucleases
MH  - Transforming Growth Factor beta/analysis
EDAT- 2000/11/09 11:00
MHDA- 2001/03/07 10:01
CRDT- 2000/11/09 11:00
PHST- 2000/11/09 11:00 [pubmed]
PHST- 2001/03/07 10:01 [medline]
PHST- 2000/11/09 11:00 [entrez]
AID - 10.2500/105065800781329573 [doi]
PST - ppublish
SO  - Am J Rhinol. 2000 Sep-Oct;14(5):279-90. doi: 10.2500/105065800781329573.