PMID- 11069714
OWN - NLM
STAT- MEDLINE
DCOM- 20010202
LR  - 20190906
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 24
IP  - 3
DP  - 2000 Nov
TI  - Construction of male and female PAC genomic libraries suitable for identification 
      of Y-chromosome-specific clones from the liverwort, Marchantia polymorpha.
PG  - 421-8
AB  - Unlike higher plants, the dioecious liverwort, Marchantia polymorpha, has 
      uniquely small sex chromosomes, with X chromosomes present only in female 
      gametophytes and Y chromosomes only in male gametophytes. We have constructed 
      respective genomic libraries for male and female plantlets using a P1-derived 
      artificial chromosome (pCYPAC2). With an average insert size of approximately 90 
      kb, each PAC library is estimated to cover the entire genome with a probability 
      of more than 99.9%. Male-specific PAC clones were screened for by differential 
      hybridization using male and female genomic DNAs as separate probes. Seventy 
      male-specific PAC clones were identified. The male specificity of one of the 
      clones, pMM4G7, was verified by Southern hybridization and PCR analysis. This 
      clone was indeed located on the Y chromosome as verified by fluorescence in situ 
      hybridization (FISH). This result shows that the Y chromosome contains unique 
      sequences that are not present either on the X chromosome or any of the 
      autosomes. Thus, the respective male and female libraries for M. polymorpha offer 
      an opportunity to identify key genes involved in the process of sex 
      differentiation and this unique system of sex determination.
FAU - Okada, S
AU  - Okada S
AD  - Laboratory of Plant Molecular Biology, Division of Integrated Life Science, 
      Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.
FAU - Fujisawa, M
AU  - Fujisawa M
FAU - Sone, T
AU  - Sone T
FAU - Nakayama, S
AU  - Nakayama S
FAU - Nishiyama, R
AU  - Nishiyama R
FAU - Takenaka, M
AU  - Takenaka M
FAU - Yamaoka, S
AU  - Yamaoka S
FAU - Sakaida, M
AU  - Sakaida M
FAU - Kono, K
AU  - Kono K
FAU - Takahama, M
AU  - Takahama M
FAU - Yamato, K T
AU  - Yamato KT
FAU - Fukuzawa, H
AU  - Fukuzawa H
FAU - Brennicke, A
AU  - Brennicke A
FAU - Ohyama, K
AU  - Ohyama K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (DNA Primers)
SB  - IM
MH  - Base Sequence
MH  - Chromosomes/genetics
MH  - DNA Primers/genetics
MH  - Genome, Plant
MH  - Genomic Library
MH  - In Situ Hybridization, Fluorescence
MH  - Nucleic Acid Hybridization
MH  - Plants/*genetics
MH  - Polymerase Chain Reaction
EDAT- 2000/11/09 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/11/09 11:00
PHST- 2000/11/09 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/11/09 11:00 [entrez]
AID - tpj882 [pii]
AID - 10.1046/j.1365-313x.2000.00882.x [doi]
PST - ppublish
SO  - Plant J. 2000 Nov;24(3):421-8. doi: 10.1046/j.1365-313x.2000.00882.x.