PMID- 11069716
OWN - NLM
STAT- MEDLINE
DCOM- 20001228
LR  - 20081121
IS  - 0008-8749 (Print)
IS  - 0008-8749 (Linking)
VI  - 204
IP  - 2
DP  - 2000 Sep 15
TI  - Differential expression and regulation of macrophage inflammatory protein 
      (MIP)-1alpha and MIP-2 genes by alveolar and peritoneal macrophages in 
      LPS-hyporesponsive C3H/HeJ mice.
PG  - 88-95
AB  - A point mutation in Toll-like receptor 4 (Tlr4) gene in C3H/HeJ mice underlies a 
      defect in LPS-induced cytokine production by peritoneal macrophages (PMphi;). 
      Whether the C-C and the C-X-C chemokines are induced differently by LPS between 
      alveolar macrophages (AMphi;) and PMphi; in this mice remains unclear. Thus, we 
      examined the expression and regulation of macrophage inflammatory protein-1alpha 
      (MIP-1alpha) and macrophage inflammatory protein-2 (MIP-2) in C3H/HeJ 
      macrophages. These results showed that the accumulation of MIP-1alpha and MIP-2 
      mRNA increased dose dependently in response to LPS. PMphi; responded to LPS to 
      produce significantly higher levels of both chemokine mRNA and protein than 
      AMphi;. In addition, both macrophages produced much more MIP-2 than MIP-1alpha by 
      the same doses of LPS stimulation. Moreover, the chemokine production by C3H/HeN 
      macrophages was significantly higher than that of the C3H/HeJ macrophages. 
      IFN-gamma suppressed the LPS-induced MIP-1alpha release but enhanced the 
      LPS-induced MIP-2 secretion in both macrophages. These results show that the 
      chemokine production was induced and regulated differentially in AMphi; and 
      PMphi;.
CI  - Copyright 2000 Academic Press.
FAU - Wang, M J
AU  - Wang MJ
AD  - Department of Education and Research, Taichung Veterans General Hospital, 
      Taichung, Taiwan, 40705, Republic of China.
FAU - Jeng, K C
AU  - Jeng KC
FAU - Shih, P C
AU  - Shih PC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Chemokine CXCL2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Monokines)
RN  - 0 (RNA, Messenger)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Chemokine CXCL2
MH  - Dose-Response Relationship, Drug
MH  - Interferon-gamma/pharmacology
MH  - Lipopolysaccharides/*immunology
MH  - Macrophage Inflammatory Proteins/*biosynthesis
MH  - Macrophages, Alveolar/*immunology
MH  - Macrophages, Peritoneal/*immunology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Monokines/*biosynthesis
MH  - RNA, Messenger/analysis
MH  - Time Factors
EDAT- 2000/11/09 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/09 11:00
PHST- 2000/11/09 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/09 11:00 [entrez]
AID - S0008-8749(00)91697-6 [pii]
AID - 10.1006/cimm.2000.1697 [doi]
PST - ppublish
SO  - Cell Immunol. 2000 Sep 15;204(2):88-95. doi: 10.1006/cimm.2000.1697.