PMID- 11069999
OWN - NLM
STAT- MEDLINE
DCOM- 20001204
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 74
IP  - 23
DP  - 2000 Dec
TI  - Characterization of transcripts expressed from human herpesvirus 6A strain GS 
      immediate-early region B U16-U17 open reading frames.
PG  - 11040-54
AB  - Several gene fragments of human herpesvirus 6 (HHV-6) have been shown to activate 
      the human immunodeficiency virus (HIV) type 1 long terminal repeat (LTR). An open 
      reading frame (ORF) designated B701 (Y. Geng, B. Chandran, S. F. Josephs, and C. 
      Wood, J. Virol. 66:1564-1570, 1992), found within a 22-kb HHV-6A strain GS 
      [HHV-6A(GS)] genomic fragment and a 3.8-kb SalI subfragment, was shown to 
      activate the HIV LTR. B701, also known as HHV-6 U16, is located in the 
      immediate-early B (IE-B) region of the genome. The sequence of the 3.8-kb genomic 
      fragment of HHV-6A(GS) is nearly identical to the published sequence of HHV-6A 
      strain U1102, with minor differences. The HHV-6A(GS) B701 ORF (U16) was used to 
      screen an HHV-6A(GS) cDNA library, and two different but overlapping cDNAs were 
      identified. These cDNAs represent differently spliced transcripts ending at 
      different polyadenylation signals. The ORFs included in the cDNAs are 
      positionally homologous to the human cytomegalovirus (HCMV) UL36 ORF. The ORF in 
      one cDNA was generated by splicing together in frame ORFs U17 and U16, and the 
      second cDNA included ORFs U16 and U15. A third differentially spliced cDNA 
      (U16+), was identified by 5' rapid amplification of cDNA ends. The predicted 
      protein was identical to the U16 portion of the U17/U16 spliced gene product but 
      did not include the U17 portion. 5'-extension analyses of the mRNAs demonstrated 
      that at least two potential transcription initiation sites were used to express 
      the transcripts encoding U17 and U16 gene products. Single-stranded U16 and U17 
      gene-specific RNA probes hybridized with at least five RNA species from infected 
      cells and demonstrated that the expression of these transcripts was 
      differentially regulated. The U17/U16 spliced gene products were expressed at IE 
      times after infection, but a multiply spliced gene product encoded by U16 was 
      expressed as a late gene. The U17/U16 and the U16+ gene products transactivated 
      the HIV LTR. Thus, while there are similarities to the HCMV UL36-UL38 gene 
      family, some of the IE-B U17/U16 transcripts are unique to HHV-6.
FAU - Flebbe-Rehwaldt, L M
AU  - Flebbe-Rehwaldt LM
AD  - Department of Microbiology, Molecular Genetics and Immunology, The University of 
      Kansas Medical Center, Kansas City, Kansas 66160, USA.
FAU - Wood, C
AU  - Wood C
FAU - Chandran, B
AU  - Chandran B
LA  - eng
SI  - GENBANK/AF294810
GR  - AI30356/AI/NIAID NIH HHS/United States
GR  - AI334502/AI/NIAID NIH HHS/United States
GR  - CA 62810/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Base Sequence
MH  - Cell Line
MH  - *Genes, Immediate-Early
MH  - HIV Long Terminal Repeat
MH  - Herpesvirus 6, Human/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Open Reading Frames
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/*analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcriptional Activation
PMC - PMC113184
EDAT- 2000/11/09 11:00
MHDA- 2001/02/28 10:01
PMCR- 2000/12/01
CRDT- 2000/11/09 11:00
PHST- 2000/11/09 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/09 11:00 [entrez]
PHST- 2000/12/01 00:00 [pmc-release]
AID - 0731 [pii]
AID - 10.1128/jvi.74.23.11040-11054.2000 [doi]
PST - ppublish
SO  - J Virol. 2000 Dec;74(23):11040-54. doi: 10.1128/jvi.74.23.11040-11054.2000.