PMID- 11070022
OWN - NLM
STAT- MEDLINE
DCOM- 20001204
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 74
IP  - 23
DP  - 2000 Dec
TI  - Structural and functional analysis of the Xestia c-nigrum granulovirus matrix 
      metalloproteinase.
PG  - 11240-6
AB  - Sequence analysis of the Xestia c-nigrum granulovirus (XcGV) genome identified an 
      open reading frame encoding a 469-amino-acid (54-kDa) protein with over 30% amino 
      acid sequence identity to a region of about 150 amino acids that includes the 
      catalytic domains of human stromelysin 1 (Str1)/matrix metalloproteinase 3 
      (MMP-3) (EC 3.4.24.17) and sea urchin hatching enzyme (HE). Stromelysin homologs 
      have not been reported from baculoviruses or other viruses. Unlike human Str1 and 
      sea urchin HE, the putative XcGV-MMP does not have a signal peptide and lacks the 
      peptide motif involved in the cysteine switch that maintains other MMPs in an 
      inactive form. The putative XcGV-MMP, however, possesses a conserved zinc-binding 
      motif in its putative catalytic domain. The XcGV-MMP homolog was cloned, and a 
      recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) that expresses XcGV-MMP 
      under the polyhedrin promoter was constructed. A distinct pattern of melanization 
      was observed in B. mori larvae infected with MMP-expressing BmNPV. Fat body 
      extracts from larvae overexpressing the 54-kDa recombinant MMP digested 
      dye-impregnated collagen (Azocoll). The enzymatic activity was inhibited by two 
      metalloproteinase inhibitors, EDTA and 1,10-phenanthroline. These results suggest 
      that the XcGV MMP-3 gene homolog encodes a functional metalloproteinase.
FAU - Ko, R
AU  - Ko R
AD  - Laboratory of Molecular Entomology and Baculovirology, RIKEN, Wako, Saitama, 
      Japan. krko@mail.ecc.u-tokyo.ac.jp
FAU - Okano, K
AU  - Okano K
FAU - Maeda, S
AU  - Maeda S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Melanins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Viral Proteins)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Baculoviridae/*enzymology
MH  - Blotting, Western
MH  - Bombyx/genetics
MH  - Fluorescent Antibody Technique
MH  - Matrix Metalloproteinase Inhibitors
MH  - Matrix Metalloproteinases/*chemistry/physiology
MH  - Melanins/metabolism
MH  - Molecular Sequence Data
MH  - Recombinant Proteins/chemistry
MH  - Structure-Activity Relationship
MH  - Viral Proteins/*chemistry
PMC - PMC113222
EDAT- 2000/11/09 11:00
MHDA- 2001/02/28 10:01
PMCR- 2000/12/01
CRDT- 2000/11/09 11:00
PHST- 2000/11/09 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/09 11:00 [entrez]
PHST- 2000/12/01 00:00 [pmc-release]
AID - 2271 [pii]
AID - 10.1128/jvi.74.23.11240-11246.2000 [doi]
PST - ppublish
SO  - J Virol. 2000 Dec;74(23):11240-6. doi: 10.1128/jvi.74.23.11240-11246.2000.