PMID- 11070155
OWN - NLM
STAT- MEDLINE
DCOM- 20010118
LR  - 20191210
IS  - 1084-8592 (Print)
IS  - 1084-8592 (Linking)
VI  - 5
IP  - 3
DP  - 2000 Sep
TI  - Automation of fluorescence in situ hybridization pretreatment: a comparative 
      study of different sample types.
PG  - 209-20
AB  - BACKGROUND: In fluorescence in situ hybridization (FISH) applications, the 
      efficiency of probe hybridization is greatly enhanced by treating the cell or 
      tissue preparation with a variety of reagents that make the target permeable 
      while preserving important morphological features. Pretreatment protocols can be 
      very labor intensive, adding cost to the test. The automation of specimen 
      pretreatment eliminates human variation in the procedure through standardization 
      of such variables as treatment times and temperatures. METHODS AND RESULTS: We 
      developed an instrument, the VP 2000 Processor, that can process up to 50 
      specimens simultaneously under computer control. In this comparative FISH study 
      of matched specimens, one set was processed according to the manual pretreatment 
      protocol and compared with specimens processed by the VP 2000. Processed specimen 
      types included paraffin-embedded breast tissue, uncultured amniocytes, bone 
      marrow, peripheral-blood lymphocytes, and uroepithelial cells recovered from 
      urine. Data show equivalent or brighter and more specific overall signal quality 
      compared with matched manually executed controls. CONCLUSIONS: The automation of 
      sample pretreatment for FISH provides a superior, more consistent level of 
      performance than the manual format.
FAU - Jacobson, K
AU  - Jacobson K
AD  - Assay Development, Vysis, Inc, Downers Grove, IL 60515, USA.
FAU - Thompson, A
AU  - Thompson A
FAU - Browne, G
AU  - Browne G
FAU - Shasserre, C
AU  - Shasserre C
FAU - Seelig, S A
AU  - Seelig SA
FAU - King, W
AU  - King W
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Mol Diagn
JT  - Molecular diagnosis : a journal devoted to the understanding of human disease 
      through the clinical application of molecular biology
JID - 9614965
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Amnion/cytology/metabolism
MH  - *Automation
MH  - Bone Marrow Cells/metabolism
MH  - Breast Neoplasms/genetics/metabolism/ultrastructure
MH  - Cell Nucleus/ultrastructure
MH  - DNA, Neoplasm/analysis
MH  - Epithelial Cells/metabolism
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*instrumentation/methods
MH  - Lymphocytes/metabolism
MH  - Paraffin Embedding
MH  - Sensitivity and Specificity
MH  - Time Factors
MH  - Urine/cytology
EDAT- 2000/11/09 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/09 11:00
PHST- 2000/11/09 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/09 11:00 [entrez]
AID - S1084-8592(00)96822-X [pii]
AID - 10.1054/modi.2000.9731 [doi]
PST - ppublish
SO  - Mol Diagn. 2000 Sep;5(3):209-20. doi: 10.1054/modi.2000.9731.