PMID- 11070502 OWN - NLM STAT- MEDLINE DCOM- 20001214 LR - 20061115 IS - 0360-4012 (Print) IS - 0360-4012 (Linking) VI - 62 IP - 4 DP - 2000 Nov 15 TI - Neurotrophic function of conditioned medium from human amniotic epithelial cells. PG - 585-90 AB - Human amniotic epithelial cells (HAEC) may have pluripotent function because they are formed from the epiblast cells at the 8th day of fertilization. Previously, we reported that HAEC have the capacity to synthesize and release acetylcholine and catecholamine associated with the binding sites of catecholamine receptors. We show the neurotrophic function of a conditioned medium from HAEC using cultured cortical neurons of E18 rats. Extensive analyses with various techniques demonstrated that HAEC and immortalized HAEC synthesize and release brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and nerve growth factor (NGF). Other neurotrophic factors were not detected in a cultured medium of HAEC by enzyme immunoassay. Various neurotrophic factors or growth factors did not show neurotrophic effects on E18 rat neuron except for EGF. Because EGF was not detected in the conditioned medium of HAEC, these data indicate an unidentified neurotrophic factor presently that is synthesized and released from HAEC. The amniotic membrane may have a significant role in supplying neurotrophic factors to the amniotic fluid as well as neurotransmitters, suggesting an important function to the early stages of neural development in the embryo. CI - Copyright 2000 Wiley-Liss, Inc. FAU - Uchida, S AU - Uchida S AD - Department of Inherited Metabolic Disease, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan. FAU - Inanaga, Y AU - Inanaga Y FAU - Kobayashi, M AU - Kobayashi M FAU - Hurukawa, S AU - Hurukawa S FAU - Araie, M AU - Araie M FAU - Sakuragawa, N AU - Sakuragawa N LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - J Neurosci Res JT - Journal of neuroscience research JID - 7600111 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Culture Media, Conditioned) RN - 0 (Glial Fibrillary Acidic Protein) RN - 0 (Microtubule-Associated Proteins) RN - 0 (Nerve Growth Factors) RN - 0 (Neuroprotective Agents) RN - 0 (Neurotrophin 3) RN - 0 (RNA, Messenger) RN - 9061-61-4 (Nerve Growth Factor) SB - IM MH - Amniotic Fluid/cytology/*metabolism MH - Animals MH - Brain-Derived Neurotrophic Factor/genetics/metabolism MH - Cell Survival/drug effects/physiology MH - Cells, Cultured/cytology/drug effects/metabolism MH - Cerebral Cortex/drug effects/embryology/metabolism MH - Culture Media, Conditioned/metabolism/*pharmacology MH - Epithelial Cells/cytology/*metabolism MH - Female MH - Fetus MH - Glial Fibrillary Acidic Protein/metabolism MH - Humans MH - Microtubule-Associated Proteins/metabolism MH - Nerve Growth Factor/genetics/metabolism MH - Nerve Growth Factors/biosynthesis/*pharmacology MH - Neurons/cytology/*drug effects/metabolism MH - Neuroprotective Agents/metabolism/*pharmacology MH - Neurotrophin 3/genetics/metabolism MH - Pregnancy MH - RNA, Messenger/metabolism MH - Rats MH - Rats, Wistar EDAT- 2000/11/09 11:00 MHDA- 2001/02/28 10:01 CRDT- 2000/11/09 11:00 PHST- 2000/11/09 11:00 [pubmed] PHST- 2001/02/28 10:01 [medline] PHST- 2000/11/09 11:00 [entrez] AID - 10.1002/1097-4547(20001115)62:4<585::AID-JNR13>3.0.CO;2-U [pii] AID - 10.1002/1097-4547(20001115)62:4<585::AID-JNR13>3.0.CO;2-U [doi] PST - ppublish SO - J Neurosci Res. 2000 Nov 15;62(4):585-90. doi: 10.1002/1097-4547(20001115)62:4<585::AID-JNR13>3.0.CO;2-U.