PMID- 11071665
OWN - NLM
STAT- MEDLINE
DCOM- 20010111
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 96
IP  - 10
DP  - 2000 Nov 15
TI  - Limited engraftment capacity of bone marrow-derived mesenchymal cells following 
      T-cell-depleted hematopoietic stem cell transplantation.
PG  - 3637-43
AB  - The engraftment capacity of bone marrow-derived mesenchymal cells was 
      investigated in 41 patients who had received a sex-mismatched, T-cell-depleted 
      allograft from human leukocyte antigen (HLA)-matched or -mismatched family 
      donors. Polymerase chain reaction (PCR) analysis of the human androgen receptor 
      (HUMARA) or the amelogenin genes was used to detect donor-derived mesenchymal 
      cells. Only 14 marrow samples (34%) from 41 consenting patients generated a 
      marrow stromal layer adequate for PCR analysis. Monocyte-macrophage contamination 
      of marrow stromal layers was reduced below the levels of sensitivity of HUMARA 
      and amelogenin assays (5% and 3%, respectively) by repeated trypsinizations and 
      treatment with the leucyl-leucine (leu-leu) methyl ester. Patients who received 
      allografts from 12 female donors were analyzed by means of the HUMARA assay, and 
      in 5 of 12 cases a partial female origin of stromal cells was demonstrated. Two 
      patients who received allografts from male donors were analyzed by amplifying the 
      amelogenin gene, and in both cases a partial male origin of stromal cells was 
      shown. Fluorescent in situ hybridization analysis using a Y probe confirmed the 
      results of PCR analysis and demonstrated in 2 cases the existence of a mixed 
      chimerism at the stromal cell level. There was no statistical difference detected 
      between the dose of fibroblast progenitors (colony-forming unit-F [CFU-F]) 
      infused to patients with donor- or host-derived stromal cells (1.18 +/- 0.13 x 
      10(4)/kg vs 1. 19 +/- 0.19 x 10(4)/kg; P >/=.97). In conclusion, marrow stromal 
      progenitors reinfused in patients receiving a T-cell-depleted allograft have a 
      limited capacity of reconstituting marrow mesenchymal cells.
FAU - Cilloni, D
AU  - Cilloni D
AD  - Department of Hematology, University of Parma, Parma, Italy.
FAU - Carlo-Stella, C
AU  - Carlo-Stella C
FAU - Falzetti, F
AU  - Falzetti F
FAU - Sammarelli, G
AU  - Sammarelli G
FAU - Regazzi, E
AU  - Regazzi E
FAU - Colla, S
AU  - Colla S
FAU - Rizzoli, V
AU  - Rizzoli V
FAU - Aversa, F
AU  - Aversa F
FAU - Martelli, M F
AU  - Martelli MF
FAU - Tabilio, A
AU  - Tabilio A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
MH  - Adult
MH  - Bone Marrow Cells/cytology
MH  - Cell Culture Techniques
MH  - Cell Division/genetics
MH  - Female
MH  - Graft Survival/*immunology
MH  - Hematopoiesis/genetics
MH  - Hematopoietic Stem Cell Transplantation/*methods/standards
MH  - Histocompatibility Testing
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Lymphocyte Depletion
MH  - Male
MH  - Mesoderm/*cytology
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/therapy
MH  - Myeloid Progenitor Cells
MH  - Polymerase Chain Reaction
MH  - Sex Factors
MH  - Stromal Cells/cytology
MH  - Tissue Donors
MH  - Transplantation Chimera/genetics
MH  - Transplantation, Homologous/methods
EDAT- 2000/11/09 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/09 11:00
PHST- 2000/11/09 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/09 11:00 [entrez]
AID - S0006-4971(20)48308-9 [pii]
PST - ppublish
SO  - Blood. 2000 Nov 15;96(10):3637-43.