PMID- 11071923
OWN - NLM
STAT- MEDLINE
DCOM- 20010202
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 11
IP  - 11
DP  - 2000 Nov
TI  - Fission yeast Int6 is not essential for global translation initiation, but 
      deletion of int6(+) causes hypersensitivity to caffeine and affects spore 
      formation.
PG  - 4005-18
AB  - Mammalian INT6 protein has been considered to be a subunit of the eukaryotic 
      translation initiation factor, eIF3. The Int6 locus is also known as a common 
      integration site of mouse mammary tumor virus (MMTV). However, the function of 
      Int6 in translation initiation and the mechanism of Int6-mediated tumor induction 
      are yet to be explored. In this study, the fission yeast, Schizosaccharomyces 
      pombe, int6(+), which is 43% identical to the mammalian counterpart, was deleted. 
      Despite the evidence that the majority of Int6 protein was associated with 40S 
      particles in this organism, strains lacking int6(+) (Deltaint6) were viable and 
      showed only moderate inhibition in the rate of in vivo global protein synthesis. 
      Polysome profile analysis showed no apparent defects in translation initiation. 
      Deltaint6 exhibited a hypersensitivity to caffeine, which could be suppressed by 
      the addition of sorbitol to the growth medium. This and other phenotypes would 
      imply that int6(+) is required for the integrity of cell membrane. In meiosis, 
      Deltaint6 produced incomplete tetrads frequently. High dosage expression of a 
      truncated mutant of int6(+) conferred a hypersensitivity to caffeine, but did not 
      cause the defect in meiosis. A possible link between the function of int6(+) and 
      the Deltaint6-phenotypes is discussed.
FAU - Bandyopadhyay, A
AU  - Bandyopadhyay A
AD  - Department of Developmental and Molecular Biology, Albert Einstein College of 
      Medicine of Yeshiva University, Jack and Pearl Resnick Campus, Bronx, New York 
      10461, USA.
FAU - Matsumoto, T
AU  - Matsumoto T
FAU - Maitra, U
AU  - Maitra U
LA  - eng
GR  - P30 CA013330/CA/NCI NIH HHS/United States
GR  - R01 GM015399/GM/NIGMS NIH HHS/United States
GR  - GM-15399/GM/NIGMS NIH HHS/United States
GR  - P30CA13330/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Eukaryotic Initiation Factor-3)
RN  - 0 (Peptide Initiation Factors)
RN  - 0 (Prokaryotic Initiation Factor-3)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Transfer, Met)
RN  - 3G6A5W338E (Caffeine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Caffeine/*pharmacology
MH  - Cell Division/genetics
MH  - Cloning, Molecular
MH  - Cytoplasm/metabolism
MH  - Eukaryotic Initiation Factor-3
MH  - Gene Deletion
MH  - Meiosis
MH  - Mice
MH  - Molecular Sequence Data
MH  - Peptide Initiation Factors/metabolism
MH  - Prokaryotic Initiation Factor-3
MH  - *Protein Biosynthesis
MH  - Proto-Oncogene Proteins/drug effects/*genetics/metabolism
MH  - RNA, Transfer, Met/metabolism
MH  - Ribosomes/metabolism
MH  - Schizosaccharomyces/*drug effects/*physiology
MH  - Sequence Homology, Amino Acid
MH  - Spores, Fungal/drug effects
PMC - PMC15053
EDAT- 2000/11/10 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/11/10 11:00
PHST- 2000/11/10 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/11/10 11:00 [entrez]
AID - 1360 [pii]
AID - 10.1091/mbc.11.11.4005 [doi]
PST - ppublish
SO  - Mol Biol Cell. 2000 Nov;11(11):4005-18. doi: 10.1091/mbc.11.11.4005.