PMID- 11072082
OWN - NLM
STAT- MEDLINE
DCOM- 20001222
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1494
IP  - 1-2
DP  - 2000 Nov 15
TI  - Genomic organization and characterization of the promoter of the human 
      ATP-binding cassette transporter-G1 (ABCG1) gene.
PG  - 175-80
AB  - The ATP-binding cassette transporter G1 (ABCG1) was recently identified as a 
      regulator of macrophage cholesterol and phospholipid transport. This transporter 
      together with ABCA1 belongs to a group of sterol-sensitive ABC proteins which are 
      induced by lipid loading or specific oxysterols. We report here the genomic 
      structure of ABCG1 along with the 5' flanking sequence using library screening 
      and BLAST search analysis. The ABCG1 gene spans more than 70 kb and contains 15 
      exons. The exon size is between 30 and 1081 bp and the introns range in size from 
      137 bp to more than 45 kb. All exon-intron boundaries display the canonical GT/AG 
      sequences. Using promoter-luciferase reporter assays in the myeloid cell lines 
      THP-1 and RAW246.7 and the hepatoma cell line HepG2 we could demonstrate the 
      functionality of the ABCG1 promoter and the minimal sequence requirements for 
      gene expression. The TATA-less proximal promoter contains multiple Sp1 binding 
      sites and a consensus sequence for sterol regulatory element binding protein.
FAU - Langmann, T
AU  - Langmann T
AD  - Institute for Clinical Chemistry and Laboratory Medicine, University of 
      Regensburg, Franz-Josef-Strauss-Allee 11, 93042, Regensburg, Germany.
FAU - Porsch-Ozcurumez, M
AU  - Porsch-Ozcurumez M
FAU - Unkelbach, U
AU  - Unkelbach U
FAU - Klucken, J
AU  - Klucken J
FAU - Schmitz, G
AU  - Schmitz G
LA  - eng
SI  - GENBANK/AJ289137
SI  - GENBANK/AJ289138
SI  - GENBANK/AJ289139
SI  - GENBANK/AJ289140
SI  - GENBANK/AJ289141
SI  - GENBANK/AJ289142
SI  - GENBANK/AJ289143
SI  - GENBANK/AJ289144
SI  - GENBANK/AJ289145
SI  - GENBANK/AJ289146
SI  - GENBANK/AJ289147
SI  - GENBANK/AJ289148
SI  - GENBANK/AJ289149
SI  - GENBANK/AJ289150
SI  - GENBANK/AJ289151
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Codon, Initiator)
RN  - 0 (RNA Splice Sites)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - ATP-Binding Cassette Transporters/*genetics
MH  - Base Sequence
MH  - Cell Line
MH  - Cloning, Molecular
MH  - Codon, Initiator/genetics
MH  - Exons/*genetics
MH  - Gene Expression Regulation
MH  - Genes, Reporter
MH  - Humans
MH  - Introns/*genetics
MH  - Molecular Sequence Data
MH  - Myeloid Cells
MH  - Promoter Regions, Genetic/*genetics
MH  - RNA Splice Sites/genetics
MH  - RNA, Messenger/analysis/genetics
MH  - Response Elements/genetics
MH  - Sequence Deletion/genetics
MH  - Tumor Cells, Cultured
EDAT- 2000/11/10 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/10 11:00
PHST- 2000/11/10 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/10 11:00 [entrez]
AID - S0167-4781(00)00215-3 [pii]
AID - 10.1016/s0167-4781(00)00215-3 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2000 Nov 15;1494(1-2):175-80. doi: 
      10.1016/s0167-4781(00)00215-3.