PMID- 11072139
OWN - NLM
STAT- MEDLINE
DCOM- 20001227
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 294
IP  - 3
DP  - 2000 Nov 24
TI  - Endomorphin and mu-opioid receptors in mouse brain mediate the analgesic effect 
      induced by 2 Hz but not 100 Hz electroacupuncture stimulation.
PG  - 159-62
AB  - This work was designed to examine whether brain endomorphins (EM1 and EM2), the 
      endogenous mu-opioid ligands, are involved in electroacupuncture (EA)-induced 
      analgesia in the mice. C57BL/6J mice were given EA for 30 min and the effect of 
      EA-induced analgesia was assessed by radiant heat tail flick latency (TFL). 
      Intracerebroventricular (i.c.v.) injection of mu-opioid receptor antagonist 
      D-Phe-Cys-Tyr-D-Tyr-Orn-Thr-Pen-Thr-NH(2) (CTOP), or antiserum against EM1 or EM2 
      was performed to see whether EA analgesia could be blocked. The results showed 
      that: (1) i.c.v. injection of CTOP at 25-100 ng dose-dependently antagonized the 
      analgesia induced by EA of 2 Hz, but not 100 Hz. (2) Intracerebroventricular 
      injection of EM1 antiserum (5 ml, 1:1 or 1:10 dilution) dose-dependently 
      antagonized 2 Hz, but not 100 Hz EA analgesia. (3) EM2 antiserum showed similar 
      effect at 1:1 dilution. The results are interpreted to mean that endogenously 
      released EM1 and EM2 and the cerebral mu-receptors are involved in mediating 2 Hz 
      but not 100 Hz EA analgesia in the mice.
FAU - Huang, C
AU  - Huang C
AD  - Neuroscience Research Institute, Peking University, 38 Xue Yuan Road, 100083, PR, 
      Beijing, China.
FAU - Wang, Y
AU  - Wang Y
FAU - Chang, J K
AU  - Chang JK
FAU - Han, J S
AU  - Han JS
LA  - eng
GR  - DA03983/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Immune Sera)
RN  - 0 (Oligopeptides)
RN  - 0 (Receptors, Opioid, mu)
RN  - 0 (endomorphin 1)
RN  - 103429-31-8 
      (phenylalanyl-cyclo(cysteinyltyrosyl-tryptophyl-ornithyl-threonyl-penicillamine)threoninamide)
RN  - 3PH5M0466G (endomorphin 2)
RN  - 51110-01-1 (Somatostatin)
SB  - IM
MH  - Animals
MH  - Brain/drug effects/physiology
MH  - *Electroacupuncture/methods
MH  - Female
MH  - Immune Sera/*pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oligopeptides/*drug effects/immunology/physiology
MH  - Pain Measurement/*drug effects
MH  - Receptors, Opioid, mu/drug effects
MH  - Somatostatin/*analogs & derivatives/pharmacology
EDAT- 2000/11/10 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/10 11:00
PHST- 2000/11/10 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/10 11:00 [entrez]
AID - S030439400001572X [pii]
AID - 10.1016/s0304-3940(00)01572-x [doi]
PST - ppublish
SO  - Neurosci Lett. 2000 Nov 24;294(3):159-62. doi: 10.1016/s0304-3940(00)01572-x.