PMID- 11073818
OWN - NLM
STAT- MEDLINE
DCOM- 20001207
LR  - 20181113
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 157
IP  - 5
DP  - 2000 Nov
TI  - Near-haploidy and subsequent polyploidization characterize the progression of 
      peripheral chondrosarcoma.
PG  - 1587-95
AB  - Chondrosarcomas are malignant cartilaginous tumors arising centrally in bone 
      (central chondrosarcoma), or secondarily within the cartilaginous cap of 
      osteochondroma (peripheral chondrosarcoma). We previously used DNA flow cytometry 
      to demonstrate that near-haploidy is relatively frequent in peripheral 
      chondrosarcomas. We performed fluorescence in situ hybridization (FISH) to 
      interphase nuclei using centromeric probes, a genome wide loss of heterozygosity 
      (LOH) analysis, and comparative genomic hybridization on five peripheral 
      chondrosarcomas. We demonstrated near-haploidy in two low-grade tumors with only 
      one copy and LOH of most chromosomes. Few chromosomes are disomic, with retention 
      of heterozygosity and overrepresentation at comparative genomic hybridization. 
      One tumor contains both a near-haploid clone with chromosomes in monosomic and 
      disomic state, and an exactly duplicated clone. Two high-grade tumors clearly 
      demonstrate polyploidization because most chromosomes show LOH and two copies at 
      FISH, whereas few chromosomes have four copies with retention of heterozygosity. 
      Using DNA from a relative, we demonstrate that chromosome loss is random 
      regardless of parental origin. Using FISH on paraffin slides, we exclude 
      near-haploidy to result from meiosis-like division in binucleated cells, 
      characteristic for chondrosarcoma. In conclusion, our results indicate that 
      near-haploidy characterizes the progression from osteochondroma toward low-grade 
      chondrosarcoma. Moreover, further progression toward high-grade chondrosarcoma is 
      characterized by polyploidization.
FAU - Bovee, J V
AU  - Bovee JV
AD  - Department of Pathology, Laboratory of Cytochemistry and Cytometry, Leiden 
      University Medical Center, Leiden, The Netherlands.
FAU - van Royen, M
AU  - van Royen M
FAU - Bardoel, A F
AU  - Bardoel AF
FAU - Rosenberg, C
AU  - Rosenberg C
FAU - Cornelisse, C J
AU  - Cornelisse CJ
FAU - Cleton-Jansen, A M
AU  - Cleton-Jansen AM
FAU - Hogendoorn, P C
AU  - Hogendoorn PC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
SB  - IM
MH  - Adult
MH  - Bone Neoplasms/*genetics/*physiopathology
MH  - Chondrosarcoma/*genetics/*physiopathology
MH  - Disease Progression
MH  - Female
MH  - *Haploidy
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Loss of Heterozygosity
MH  - Male
MH  - Microsatellite Repeats
MH  - Middle Aged
MH  - Nucleic Acid Hybridization
MH  - *Ploidies
PMC - PMC1885743
EDAT- 2000/11/14 11:00
MHDA- 2001/02/28 10:01
PMCR- 2001/05/01
CRDT- 2000/11/14 11:00
PHST- 2000/11/14 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/14 11:00 [entrez]
PHST- 2001/05/01 00:00 [pmc-release]
AID - S0002-9440(10)64796-7 [pii]
AID - 2386 [pii]
AID - 10.1016/S0002-9440(10)64796-7 [doi]
PST - ppublish
SO  - Am J Pathol. 2000 Nov;157(5):1587-95. doi: 10.1016/S0002-9440(10)64796-7.