PMID- 11074472
OWN - NLM
STAT- MEDLINE
DCOM- 20010125
LR  - 20191210
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 62
IP  - 4
DP  - 2000 Dec
TI  - Comparative quantification of diverse serotypes of HIV-1 in plasma from a diverse 
      population of patients.
PG  - 445-9
AB  - HIV-1 is characterised by extensive genetic variability encompassing at least 10 
      different phylogenetically related clades within the major group of HIV-1 
      subtypes. Most commercially available HIV-1 RNA plasma viral load assays have 
      been optimised with clade B viruses and may yield misleadingly low RNA levels for 
      nonclade B viruses that are increasingly found in Europe. In this study we 
      compare the most recent versions of the Roche Amplicor HIV Monitor and the Chiron 
      Quantiplex for ability to detect viraemia in a population of patients infected 
      with a range of HIV-1 subtypes. EDTA-treated plasma was obtained from 206 
      patients. The Amplicor and Quantiplex assays were carried out in accordance with 
      manufacturers' instructions. Results from 53/206 (25.7%) samples differed by >0.4 
      log between Amplicor 1.5 and Quantiplex 3.0. A >0.5 log and 1.0 log difference 
      was detected between Amplicor 1.5 and Quantiplex 3.0 in 37/206 (17.9%) and 7/206 
      (3.4%) of samples, respectively. Overall, Amplicor 1.5 gave a median value of 
      0.22 log higher than Quantiplex 3.0. Discordant results were detected in 53 out 
      of 206 (25.7%) samples. Of these 22 out of 123 (17.9%) samples were of UK origin, 
      18 out of 43 (41.9%) African, 1 out of 8 (12.5%) South American, 1 out of 6 
      (16.7%) North American, 4 out of 9 (44.4%) North European, 3 out of 11 (23.7%) 
      South European and 3 out of 7 (42.3%) Asian samples, respectively. Serotyping 
      revealed that discordant viral load results between Amplicor 1.5 and Quantiplex 
      3.0 occurred within samples from all subtypes (A-E). Despite the improvements 
      made to both the Roche Amplicor and the Chiron Quantiplex assays discordant 
      results were detected between the two assays in 25.7% of cases. In a substantial 
      minority of patients there were major discrepancies between the two assays that 
      were not explained by HIV subtype differences.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Clarke, J R
AU  - Clarke JR
AD  - Department of Genitourinary Medicine and Communicable Diseases, Jefferiss 
      Research Trust Laboratories, Division of Medicine, Imperial College School of 
      Medicine at St. Mary's Hospital, London, United Kingdom. j.r.clarke@ic.ac.uk
FAU - Galpin, S
AU  - Galpin S
FAU - Braganza, R
AU  - Braganza R
FAU - Ashraf, A
AU  - Ashraf A
FAU - Russell, R
AU  - Russell R
FAU - Churchill, D R
AU  - Churchill DR
FAU - Weber, J N
AU  - Weber JN
FAU - McClure, M O
AU  - McClure MO
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (RNA, Viral)
RN  - 0 (Reagent Kits, Diagnostic)
SB  - IM
MH  - HIV Infections/blood/drug therapy/*virology
MH  - HIV-1/classification/genetics/*isolation & purification
MH  - Humans
MH  - RNA, Viral/*blood
MH  - *Reagent Kits, Diagnostic
MH  - Reproducibility of Results
MH  - Serotyping
MH  - Viral Load
EDAT- 2000/11/14 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/14 11:00
PHST- 2000/11/14 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/14 11:00 [entrez]
AID - 10.1002/1096-9071(200012)62:4<445::AID-JMV8>3.0.CO;2-N [pii]
AID - 10.1002/1096-9071(200012)62:4<445::aid-jmv8>3.0.co;2-n [doi]
PST - ppublish
SO  - J Med Virol. 2000 Dec;62(4):445-9. doi: 
      10.1002/1096-9071(200012)62:4<445::aid-jmv8>3.0.co;2-n.