PMID- 11074557
OWN - NLM
STAT- MEDLINE
DCOM- 20001212
LR  - 20190822
IS  - 0361-8609 (Print)
IS  - 0361-8609 (Linking)
VI  - 65
IP  - 4
DP  - 2000 Dec
TI  - Characterization of complex chromosomal abnormalities in B-cell lymphoma by a 
      combined spectral karyotyping (SKY) analysis and fluorescence in situ 
      hybridization (FISH) using a 14q telomere probe.
PG  - 291-7
AB  - We report a case of non-Hodgkin's lymphoma of unknown origin with invasion into 
      bone marrow and brain. This case showed complex chromosomal abnormalities, 
      including five clonal marker chromosomes (mar) and four additional materials of 
      unknown origin (add) that could not be identified by means of conventional 
      G-banding. Spectral karyotyping (SKY) analysis could not only determine the 
      origin and organization of all thus far unidentified structural chromosomal 
      abnormalities but also detect two cryptic unbalanced translocations, which had 
      been erroneously considered to be normal on the basis of G-banding analysis, and 
      correct one abnormality misidentified by G banding. Among these abnormalities, we 
      identified the new partner site of the 14q32 translocation, 22q13, and the 
      jumping translocations involving 2p23 as a new donor chromosome. Furthermore, by 
      using fluorescence in situ hybridization (FISH) with the probes specific for the 
      14q telomere, we could identify the unbalanced translocation of t(3;14)(q27;q32), 
      which had been erroneously considered to be normal chromosome 3 on the basis of 
      not only G-banding but also of SKY analysis. This translocation is one of the 
      most frequent chromosomal abnormalities in B-cell lymphoma, especially diffuse 
      large cell lymphoma. After SKY and FISH analysis, the original descriptions in 
      the G-band karyotype were modified for a total of 13 chromosomes. The combination 
      of SKY and FISH using the 14q telomere probe was therefore considered very useful 
      for the characterization of complex cytogenetic cases in B-cell lymphoma.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Kakazu, N
AU  - Kakazu N
AD  - Department of Hygiene, Kyoto Prefectural University of Medicine, Kyoto, Japan.
FAU - Kito, K
AU  - Kito K
FAU - Hitomi, T
AU  - Hitomi T
FAU - Oita, J
AU  - Oita J
FAU - Nishida, K
AU  - Nishida K
FAU - Masuda, K
AU  - Masuda K
FAU - Miki, T
AU  - Miki T
FAU - Abe, T
AU  - Abe T
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Hematol
JT  - American journal of hematology
JID - 7610369
SB  - IM
MH  - *Chromosome Aberrations
MH  - *Chromosome Disorders
MH  - *Chromosomes, Human, Pair 14
MH  - *Chromosomes, Human, Pair 2
MH  - *Chromosomes, Human, Pair 22
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Lymphoma, B-Cell/*genetics/pathology/ultrastructure
MH  - Middle Aged
EDAT- 2000/11/14 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/14 11:00
PHST- 2000/11/14 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/14 11:00 [entrez]
AID - 10.1002/1096-8652(200012)65:4<291::AID-AJH7>3.0.CO;2-7 [pii]
AID - 10.1002/1096-8652(200012)65:4<291::aid-ajh7>3.0.co;2-7 [doi]
PST - ppublish
SO  - Am J Hematol. 2000 Dec;65(4):291-7. doi: 
      10.1002/1096-8652(200012)65:4<291::aid-ajh7>3.0.co;2-7.