PMID- 11075944
OWN - NLM
STAT- MEDLINE
DCOM- 20010222
LR  - 20091119
IS  - 0145-5680 (Print)
IS  - 0145-5680 (Linking)
VI  - 46
IP  - 7
DP  - 2000 Nov
TI  - Integration of proviral sequences, but not at the common integration sites of the 
      FGF8 locus, in an androgen-dependent mouse mammary Shionogi carcinoma.
PG  - 1147-56
AB  - A retroviral insertional mutation, especially by mouse mammary tumor virus 
      (MMTV), is a major cause of murine mammary tumorigenesis. Prompted by our 
      previous finding that FGF8, an insertionally activated cellular oncogene, is 
      highly expressed in androgen-dependent mouse mammary Shionogi carcinoma cells, we 
      here investigated retroviral integration adjacent to the fgf8 locus in Shionogi 
      carcinoma. In the genomic Southern blots for fgf8 and its 5'-upstream gene npm3, 
      the hybridized fragments were identical to the host DD/Sio mice, the original 
      Shionogi carcinoma 115 tumor, and a pair of cultured Shionogi carcinoma cell 
      lines of SC-3 and SC-4, suggesting that no retroviral integration occurred around 
      either loci. The genomic cloning for the fgf8 locus from SC-3 cells also 
      confirmed no MMTV integration. In addition, npm3, which is usually coactivated 
      with fgf8 by MMTV insertion,was not up-regulated by androgens in SC-3 cells. All 
      these findings led us to conclude that no retroviral insertion was present at the 
      common integration sites adjacent to the fgf8 locus in Shionogi carcinoma 
      although we demonstrated in this study that multiple proviral sequences of MMTV, 
      Moloney murine sarcoma virus and FBJ-murine sarcoma virus are integrated into 
      SC-3 cells in association with their distinct promoter activity in SC-3 cells.
FAU - Kuriki, K
AU  - Kuriki K
AD  - Department of Pathology, Jichi Medical School, Kawachi, Tochigi, Japan.
FAU - Kamiakito, T
AU  - Kamiakito T
FAU - Yoshida, H
AU  - Yoshida H
FAU - Saito, K
AU  - Saito K
FAU - Fukayama, M
AU  - Fukayama M
FAU - Tanaka, A
AU  - Tanaka A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (Androgens)
RN  - 0 (Fgf8 protein, mouse)
RN  - 0 (Npm3 protein, mouse)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Nucleoplasmins)
RN  - 0 (Phosphoproteins)
RN  - 148997-75-5 (Fibroblast Growth Factor 8)
RN  - 62031-54-3 (Fibroblast Growth Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Androgens/*pharmacology
MH  - Animals
MH  - Blotting, Southern
MH  - DNA Mutational Analysis
MH  - Fibroblast Growth Factor 8
MH  - Fibroblast Growth Factors/*genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Mammary Neoplasms, Animal/chemically induced/*genetics/*virology
MH  - Mammary Tumor Virus, Mouse/chemistry/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis, Insertional/genetics
MH  - Nuclear Proteins/genetics
MH  - Nucleoplasmins
MH  - Phosphoproteins/genetics
MH  - Restriction Mapping
MH  - Retroviridae Infections/chemically induced/genetics/virology
MH  - Sequence Alignment
MH  - Terminal Repeat Sequences/genetics
MH  - Tumor Cells, Cultured
MH  - Tumor Virus Infections/chemically induced/genetics/virology
MH  - Virus Integration/*genetics
EDAT- 2000/11/15 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/11/15 11:00
PHST- 2000/11/15 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/11/15 11:00 [entrez]
PST - ppublish
SO  - Cell Mol Biol (Noisy-le-grand). 2000 Nov;46(7):1147-56.