PMID- 11076027
OWN - NLM
STAT- MEDLINE
DCOM- 20010621
LR  - 20051116
IS  - 1431-6730 (Print)
IS  - 1431-6730 (Linking)
VI  - 381
IP  - 9-10
DP  - 2000 Sep-Oct
TI  - Protein-protein interactions in receptor activation and intracellular signalling.
PG  - 955-9
AB  - We review here signalling complexes that we have defined using X-ray analysis in 
      our laboratory. They include growth factors and their receptors: nerve growth 
      factor (NGF) and its hetero-hexameric 7S NGF storage complex, hepatocyte growth 
      factor/scatter factor (HGF/SF) NK1 dimers and fibroblast growth factor (FGF1) in 
      complex with its receptor (FGFR2) ectodomain and heparin. We also review our 
      recent structural studies on intracellular signalling complexes, focusing on 
      phosducin transducin GPry, CK2 protein kinase and its complexes, and the cyclin 
      D-dependent kinase, Cdk6, bound to the cell cycle inhibitor p19INK4d. Comparing 
      the structures of these complexes with others we show that the surface area 
      buried in signalling interactions does not always give a good indication of the 
      strength of the interactions. We show that conformational changes are often 
      important in complexes with intermediate buried surface areas of 1500 to 2000 A2, 
      such as Cdk6INK4 interactions. Some interactions involve recognition of 
      continuous epitopes, where there is no necessity for a tertiary structure and 
      very often the binding conformation is induced during the process of interaction, 
      for example phosducin binding to the betagamma subunits (Gtbetagamma) of the 
      heterotrimeric G protein transducin.
FAU - Blundell, T L
AU  - Blundell TL
AD  - Department of Biochemistry, University of Cambridge, UK.
FAU - Burke, D F
AU  - Burke DF
FAU - Chirgadze, D
AU  - Chirgadze D
FAU - Dhanaraj, V
AU  - Dhanaraj V
FAU - Hyvonen, M
AU  - Hyvonen M
FAU - Innis, C A
AU  - Innis CA
FAU - Parisini, E
AU  - Parisini E
FAU - Pellegrini, L
AU  - Pellegrini L
FAU - Sayed, M
AU  - Sayed M
FAU - Sibanda, B L
AU  - Sibanda BL
LA  - eng
SI  - PDB/1BET
PT  - Journal Article
PT  - Review
PL  - Germany
TA  - Biol Chem
JT  - Biological chemistry
JID - 9700112
RN  - 0 (Proteins)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Biotransformation
MH  - Cell Communication/*physiology
MH  - Humans
MH  - Proteins/chemistry/*physiology
MH  - Receptors, Cell Surface/*physiology
MH  - Signal Transduction/*physiology
RF  - 18
EDAT- 2000/11/15 11:00
MHDA- 2001/06/22 10:01
CRDT- 2000/11/15 11:00
PHST- 2000/11/15 11:00 [pubmed]
PHST- 2001/06/22 10:01 [medline]
PHST- 2000/11/15 11:00 [entrez]
AID - 10.1515/BC.2000.117 [doi]
PST - ppublish
SO  - Biol Chem. 2000 Sep-Oct;381(9-10):955-9. doi: 10.1515/BC.2000.117.