PMID- 11076996
OWN - NLM
STAT- MEDLINE
DCOM- 20010208
LR  - 20190513
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 15
IP  - 6
DP  - 2000 Nov
TI  - Chromosome painting reveals specific patterns of chromosome occurrence in 
      mitomycin C- and diethylstilboestrol-induced micronuclei.
PG  - 459-67
AB  - Cultures of human blood lymphocytes from three subjects were incubated with the 
      clastogen mitomycin C (MMC, 500 ng/ml) and the aneugen diethylstilboestrol (DES, 
      80 microM) 23 h before harvesting, to induce formation of micronuclei (MN) and 
      numerical and structural alterations in metaphase chromosomes. We used 
      fluorescence in situ hybridization (FISH) with painting probes for all human 
      chromosomes to determine which chromosomes had contributed material to the 
      induced MN. MMC treatment induced an approximately 18-fold increase in MN and led 
      to a significant increase in hypodiploidy and structural chromosome aberrations 
      in metaphase preparations. Undercondensation of pericentromeric heterochromatin 
      of chromosomes 9 and 1 occurred in 20-75% of metaphases and FISH disclosed an 
      abundance of material from these chromosomes in induced MN (62-69% from 
      chromosome 9 and 7-12% from chromosome 1). DES treatment of lymphocytes induced a 
      seven-fold increase in MN frequency and four-fold increase in the frequency of 
      numerical aberrations; structural aberrations were not significantly increased. 
      FISH analysis showed that material from all chromosomes was present in 
      DES-induced MN, with material from chromosome 1 present in 16% of MN and material 
      from each other chromosomes being present in 2-10% of MN. Material from 
      chromosomes 14, 19 and 21 was significantly more frequent material from 
      chromosome Y significantly less frequent in DES-treated cells than in controls. 
      The findings of the MMC studies indicate that the heterochromatin block of 
      chromosome 9 is a specific target for MMC-induced undercondensation, which 
      induces a preferential occurrence of chromosome 9 material in MN. DES, in 
      contrast, does not trigger heterochromatin decondensation and fails to induce 
      such a significant appearance of material of particular chromosomes in MN.
FAU - Fauth, E
AU  - Fauth E
AD  - Abt. Humanbiologie und Humangenetik der Universitat, Postfach 3049, D-67653 
      Kaiserslautern, Germany. efauth@rhrk.uni-kl.de
FAU - Scherthan, H
AU  - Scherthan H
FAU - Zankl, H
AU  - Zankl H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (Antineoplastic Agents, Hormonal)
RN  - 0 (Carcinogens)
RN  - 0 (Heterochromatin)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
RN  - 50SG953SK6 (Mitomycin)
RN  - 731DCA35BT (Diethylstilbestrol)
SB  - IM
MH  - Adult
MH  - Antibiotics, Antineoplastic/*pharmacology
MH  - Antineoplastic Agents, Hormonal/*pharmacology
MH  - Carcinogens
MH  - Cells, Cultured
MH  - Chromosome Aberrations
MH  - *Chromosome Painting
MH  - Chromosomes, Human, Pair 1/drug effects
MH  - Chromosomes, Human, Pair 9/drug effects
MH  - Diethylstilbestrol/pharmacology
MH  - Female
MH  - Heterochromatin/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Indoles/pharmacology
MH  - Lymphocytes/drug effects
MH  - Male
MH  - Micronuclei, Chromosome-Defective/*genetics
MH  - Mitomycin/*pharmacology
MH  - Mutation
MH  - Plasmids/metabolism
MH  - Ploidies
EDAT- 2000/11/15 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/11/15 11:00
PHST- 2000/11/15 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/11/15 11:00 [entrez]
AID - 10.1093/mutage/15.6.459 [doi]
PST - ppublish
SO  - Mutagenesis. 2000 Nov;15(6):459-67. doi: 10.1093/mutage/15.6.459.