PMID- 11077004
OWN - NLM
STAT- MEDLINE
DCOM- 20010208
LR  - 20190513
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 15
IP  - 6
DP  - 2000 Nov
TI  - Increased cytogenetic damage detected by FISH analysis on micronuclei in 
      peripheral lymphocytes from alcoholics.
PG  - 517-23
AB  - Alcohol abuse greatly increases the risk of various malignancies, including 
      cancer of the liver and digestive tract. Although it is thought that this may be 
      due, at least partially, to the mutagenic properties of ethanol, little is known 
      about the genotoxic effects of ethanol in humans. We investigated the chromosomal 
      damage in lymphocytes from 20 alcoholics and 20 controls using the micronucleus 
      (MN) assay combined with fluorescence in situ hybridization (FISH) with a 
      pancentromeric DNA probe capable of differentiating centromere positive (C+) from 
      centromere negative (C-) MN. The frequency of MN in binucleate lymphocytes was 
      significantly higher in alcoholics than in controls (12.0 +/- 5.4 and 7.6 +/- 
      1.6, respectively; P: < 0.05). FISH revealed significantly higher frequencies of 
      C+ MN in alcoholics than in controls (8.2 +/- 4.8 and 3.4 +/- 1.4, respectively; 
      P: < 0.05). In the alcoholics, no association was found between years of alcohol 
      abuse and frequency of MN or C+ MN. However, age influenced MN and C+ MN 
      frequency both in alcoholics and controls. These results indicate that alcohol 
      abuse may well induce chromosome loss in humans, suggesting a possible aneugenic 
      mechanism of alcohol. This effect could contribute to the health hazards related 
      to alcoholism such as cancer risk.
FAU - Maffei, F
AU  - Maffei F
AD  - Department of Pharmacology, University of Bologna, Via Irnerio 48, 40126 Bologna, 
      Italy. fmaffei@biocfarm.unibo.it
FAU - Fimognari, C
AU  - Fimognari C
FAU - Castelli, E
AU  - Castelli E
FAU - Stefanini, G F
AU  - Stefanini GF
FAU - Forti, G C
AU  - Forti GC
FAU - Hrelia, P
AU  - Hrelia P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 3CHI920QS7 (Cytochalasin B)
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Adult
MH  - Age Factors
MH  - *Alcohol Drinking
MH  - Alcoholism/*genetics
MH  - Case-Control Studies
MH  - Cell Nucleus/metabolism
MH  - Cells, Cultured
MH  - Centromere/*metabolism
MH  - Chromosome Aberrations/*genetics
MH  - Cytochalasin B/pharmacology
MH  - *DNA Damage
MH  - *Ethanol
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Lymphocytes/*metabolism
MH  - Male
MH  - Micronuclei, Chromosome-Defective/*metabolism
MH  - Middle Aged
MH  - Smoking
MH  - Time Factors
EDAT- 2000/11/15 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/11/15 11:00
PHST- 2000/11/15 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/11/15 11:00 [entrez]
AID - 10.1093/mutage/15.6.517 [doi]
PST - ppublish
SO  - Mutagenesis. 2000 Nov;15(6):517-23. doi: 10.1093/mutage/15.6.517.