PMID- 11077420
OWN - NLM
STAT- MEDLINE
DCOM- 20010104
LR  - 20190906
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 428
IP  - 4
DP  - 2000 Dec 25
TI  - BDNF abolishes the survival effect of NT-3 in axotomized Clarke neurons of adult 
      rats.
PG  - 671-80
AB  - Neurotrophin-3 (NT-3) and brain-derived neurotrophic factor (BDNF) have 
      previously been shown to support survival and axonal regeneration in various 
      types of neurons. Also, synergistic neuroprotective effects of these 
      neurotrophins have been reported in descending rubrospinal neurons after cervical 
      spinal cord injury (Novikova et al., [2000] Eur. J. Neurosci. 12:776-780). The 
      present study investigates the effects of intrathecally delivered NT-3 and BDNF 
      on the survival and atrophy of ascending spinocerebellar neurons of Clarke 
      nucleus (CN) after cervical spinal cord injury in adult rats. At 8 weeks after 
      cervical spinal cord hemisection, 40% of the axotomized CN neurons had been lost, 
      and the remaining cells exhibited marked atrophy. Microglial activity was 
      significantly increased in CN of the operated side. Intrathecal infusion of NT-3 
      for 8 weeks postoperatively resulted in 91% cell survival and a reduction in cell 
      atrophy, but did not reduce microglial activity. In spite of the fact that the CN 
      neurons expressed both TrkC and TrkB receptors, only NT-3 had a neuroprotective 
      effect, whereas BDNF was ineffective. Furthermore, when a combination of BDNF and 
      NT-3 was administered, the neuroprotective effect of NT-3 was lost. The present 
      results indicate a therapeutic potential for NT-3 in the treatment of spinal cord 
      injury, but also demonstrate that in certain neuronal populations the 
      neuroprotection obtained by a combination of neurotrophic factors may be less 
      than that of a single neurotrophin.
CI  - Copyright 2000 Wiley-Liss, Inc.
FAU - Novikova, L N
AU  - Novikova LN
AD  - Department of Integrative Medical Biology, Section for Anatomy, Umea University, 
      SE-901 87 Umea, Sweden.
FAU - Novikov, L N
AU  - Novikov LN
FAU - Kellerth, J O
AU  - Kellerth JO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Neurotrophin 3)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
SB  - IM
MH  - Animals
MH  - Axotomy/*adverse effects
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Survival/*drug effects/physiology
MH  - Cerebellum/drug effects/pathology/physiopathology
MH  - Drug Interactions/*physiology
MH  - Female
MH  - Microglia/cytology/physiology
MH  - Neural Pathways/drug effects/pathology/physiopathology
MH  - Neuroprotective Agents/pharmacology
MH  - Neurotrophin 3/*pharmacology
MH  - Nitric Oxide Synthase/metabolism
MH  - Rats/anatomy & histology/*metabolism
MH  - Rats, Sprague-Dawley
MH  - Retrograde Degeneration/*drug therapy/pathology/physiopathology
MH  - Spinal Cord/drug effects/pathology/physiopathology
MH  - Spinal Cord Injuries/*drug therapy/pathology/physiopathology
EDAT- 2000/11/15 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/15 11:00
PHST- 2000/11/15 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/15 11:00 [entrez]
AID - 10.1002/1096-9861(20001225)428:4<671::AID-CNE7>3.0.CO;2-H [pii]
AID - 10.1002/1096-9861(20001225)428:4<671::aid-cne7>3.0.co;2-h [doi]
PST - ppublish
SO  - J Comp Neurol. 2000 Dec 25;428(4):671-80. doi: 
      10.1002/1096-9861(20001225)428:4<671::aid-cne7>3.0.co;2-h.