PMID- 11079471
OWN - NLM
STAT- MEDLINE
DCOM- 20010531
LR  - 20220408
IS  - 1011-1344 (Print)
IS  - 1011-1344 (Linking)
VI  - 56
IP  - 2-3
DP  - 2000 Jul
TI  - UV-A breakage sensitivity of human chromosomes as measured by COMET-FISH depends 
      on gene density and not on the chromosome size.
PG  - 109-17
AB  - COMET-FISH, a single cell-based combination of COMET-assay (also known as single 
      cell gel electrophoresis (SCGE)) with fluorescence in situ hybridization (FISH) 
      allows region specific studies on DNA stability and damage. COMET-FISH can be 
      used to investigate UV-A-induced DNA damage of selected whole chromosomes. In the 
      present work, a modified COMET-FISH protocol with whole chromosome painting 
      probes was used to study whether UV-A-induced DNA damage is distributed randomly 
      over the whole genome or occurs at preferred sites. The study was performed with 
      12 different chromosome painting probes (for chromosomes 1, 2, 3, 8, 9, 11, 14, 
      18, 19, 21, X and Y). The results on human lymphocytes irradiated with 500 kJ/m2 
      at a wavelength of 365 nm indicate that the induced number of chromatin strand 
      breaks does not correlate with the chromosome size. They therefore are 
      distributed in a non-random manner. For example, fragments of the gene-rich 
      chromosome chromosome 1 were found in the comet tail in only 3% of the examined 
      cells, and thus chromosome 1 is rather stable, whereas fragmentation of the 
      gene-poor chromosome 8 was observed in 25% of all comets. On the basis of all 12 
      chromosomes analyzed, an inverse correlation between the density of active genes 
      and the sensitivity toward UV-A radiation is found.
FAU - Rapp, A
AU  - Rapp A
AD  - Institut fur Molekulare Biotechnologie, Jena, Germany. bar@imb-jena.de
FAU - Bock, C
AU  - Bock C
FAU - Dittmar, H
AU  - Dittmar H
FAU - Greulich, K O
AU  - Greulich KO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - J Photochem Photobiol B
JT  - Journal of photochemistry and photobiology. B, Biology
JID - 8804966
RN  - 0 (Chromatin)
SB  - IM
MH  - Cells, Cultured
MH  - Chromatin/radiation effects
MH  - Chromosome Mapping
MH  - Chromosome Painting/methods
MH  - Chromosomes, Human/genetics/*radiation effects/ultrastructure
MH  - Comet Assay
MH  - *DNA Damage
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Lymphocytes/*radiation effects
MH  - Male
MH  - *Ultraviolet Rays
EDAT- 2000/11/18 11:00
MHDA- 2001/06/02 10:01
CRDT- 2000/11/18 11:00
PHST- 2000/11/18 11:00 [pubmed]
PHST- 2001/06/02 10:01 [medline]
PHST- 2000/11/18 11:00 [entrez]
AID - S1011-1344(00)00052-X [pii]
AID - 10.1016/s1011-1344(00)00052-x [doi]
PST - ppublish
SO  - J Photochem Photobiol B. 2000 Jul;56(2-3):109-17. doi: 
      10.1016/s1011-1344(00)00052-x.