PMID- 11081462
OWN - NLM
STAT- MEDLINE
DCOM- 20010308
LR  - 20081121
IS  - 1386-0291 (Print)
IS  - 1386-0291 (Linking)
VI  - 22
IP  - 4
DP  - 2000
TI  - Leukocyte activation, oxidant stress and red blood cell properties after acute, 
      exhausting exercise in rats.
PG  - 253-9
AB  - Red blood cell (RBC) mechanical alterations and oxidative damage were 
      investigated after an acute exhausting exercise in rats, together with the 
      leukocyte activation. The groups formed as follows. Control (C) (n = 9), group I 
      (n = 9) and group II (n = 7) from which blood samples were collected 15 minutes 
      and 24 hours respectively, after acute exercise. The rats were subjected to 
      running at a speed of 17 m/min until exhaustion. The leukocyte phagocytic 
      activity (LPA), RBC lipid peroxidation and RBC deformability were measured. LPA 
      increased significantly after the exhausting exercise and prolonged till 24 hours 
      (p = 0.0168). RBC membrane lipid peroxidation was gradually increased till 24 
      hours (p = 0.0297) and there was a significant correlation between LPA and RBC 
      lipid peroxidation (r = 0.63, p = 0.015). There was a slight but significant 
      decrease in mean corpuscular volume (MCV) (p = 0.0467) and increase in mean 
      corpuscular hemoglobin concentration (MCHC) (p = 0.0458) suggesting a cellular 
      dehydration after 24 hours. No significant alteration was detected in RBC 
      deformability, assessed by the Cell Transit Analyzer (CTA) and thought that 
      decreased MCV might have masked to determine the alterations in membrane 
      mechanical properties in CTA. As a conclusion the results imply that activated 
      leukocytes might play role in the RBC damage observed after exhausting exercise 
      encouraging oxidative stress.
FAU - Temiz, A
AU  - Temiz A
AD  - Department of Biophysics, School of Medicine, Dokuz Eylul University, 
      Balcova-Izmir, Turkey. aysegul.temiz@deu.edu.tr
FAU - Baskurt, O K
AU  - Baskurt OK
FAU - Pekcetin, C
AU  - Pekcetin C
FAU - Kandemir, F
AU  - Kandemir F
FAU - Gure, A
AU  - Gure A
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Clin Hemorheol Microcirc
JT  - Clinical hemorheology and microcirculation
JID - 9709206
RN  - 0 (Hemoglobins)
RN  - 0 (Membrane Lipids)
RN  - 0 (Thiobarbituric Acid Reactive Substances)
SB  - IM
MH  - Animals
MH  - Erythrocyte Count
MH  - Erythrocyte Deformability
MH  - Erythrocyte Indices
MH  - Erythrocyte Membrane/chemistry
MH  - Hematocrit
MH  - Hemoglobins/analysis
MH  - Lipid Peroxidation
MH  - *Lymphocyte Activation
MH  - Male
MH  - Membrane Lipids/blood/chemistry
MH  - Oxidative Stress
MH  - Oxygen Consumption
MH  - Phagocytosis
MH  - *Physical Exertion
MH  - Rats
MH  - Rats, Wistar
MH  - Running
MH  - Thiobarbituric Acid Reactive Substances/analysis
EDAT- 2000/11/18 11:00
MHDA- 2001/03/10 10:01
CRDT- 2000/11/18 11:00
PHST- 2000/11/18 11:00 [pubmed]
PHST- 2001/03/10 10:01 [medline]
PHST- 2000/11/18 11:00 [entrez]
PST - ppublish
SO  - Clin Hemorheol Microcirc. 2000;22(4):253-9.