PMID- 11081860
OWN - NLM
STAT- MEDLINE
DCOM- 20001129
LR  - 20190628
IS  - 0003-4975 (Print)
IS  - 0003-4975 (Linking)
VI  - 70
IP  - 4
DP  - 2000 Oct
TI  - Contractile smooth muscle cell apoptosis early after saphenous vein grafting.
PG  - 1145-53
AB  - BACKGROUND: The media of saphenous veins is composed of two cell populations: 
      smooth muscle (SMC) and non-smooth muscle (NSMC) cells. Previous studies 
      demonstrate a loss of SMCs by 3 days after grafting, despite an increase in cell 
      proliferation. The purpose of this study is to determine the rates of apoptotic 
      cell death versus cell proliferation for the two major cell populations of the 
      media. METHODS: Veins (six/time point) were examined at 0.5, 1, 2, 4, 8, 24, and 
      48 hours after grafting in crossbred pigs. Terminal transferase-mediated dUTP 
      nick end labeling (TUNEL) and proliferating cell nuclear antigen (PCNA) stains 
      were used to assess apoptosis and proliferation. Apoptosis was also corroborated 
      with confocal and electron microscopy. RESULTS: Apoptosis was high in both cell 
      populations: at 8 hours, SMC and NSMC apoptosis peaked at 14.5% +/- 3.5% and 
      49.9% +/- 7.8%, respectively. In contrast, cell proliferation was different 
      between the two populations. SMC proliferation was low at all time points, 
      whereas NSMC proliferation rose to 22% +/- 5.4% by 48 hours. CONCLUSIONS: Medial 
      SMCs are removed through apoptosis and appear to be replaced by fibrous tissue 
      (NSMCs) early after vein grafting. This reciprocal change between the medial SMC 
      and NSMC populations may contribute to late vein graft degeneration.
FAU - Rodriguez, E
AU  - Rodriguez E
AD  - Department of Surgery, Thomas Jefferson University Hospital, Philadelphia, 
      Pennsylvania, USA.
FAU - Lambert, E H
AU  - Lambert EH
FAU - Magno, M G
AU  - Magno MG
FAU - Mannion, J D
AU  - Mannion JD
LA  - eng
GR  - HL6067201/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Ann Thorac Surg
JT  - The Annals of thoracic surgery
JID - 15030100R
RN  - 0 (Proliferating Cell Nuclear Antigen)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Carotid Stenosis/pathology/*surgery
MH  - Cell Death/physiology
MH  - Cell Division/physiology
MH  - In Situ Nick-End Labeling
MH  - Microscopy, Confocal
MH  - Microscopy, Electron
MH  - Muscle, Smooth, Vascular/*pathology
MH  - Postoperative Complications/*pathology
MH  - Proliferating Cell Nuclear Antigen/analysis
MH  - Saphenous Vein/pathology/*transplantation
MH  - Swine
MH  - Tunica Media/pathology
EDAT- 2000/11/18 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/18 11:00
PHST- 2000/11/18 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/18 11:00 [entrez]
AID - S0003-4975(00)01768-9 [pii]
AID - 10.1016/s0003-4975(00)01768-9 [doi]
PST - ppublish
SO  - Ann Thorac Surg. 2000 Oct;70(4):1145-53. doi: 10.1016/s0003-4975(00)01768-9.