PMID- 11081964
OWN - NLM
STAT- MEDLINE
DCOM- 20010301
LR  - 20220318
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 43
IP  - 5
DP  - 2000 Oct
TI  - Molecular cytogenetic analysis of intergeneric chromosomal translocations between 
      wheat (Triticum aestivum L.) and Dasypyrum villosum arising from tissue culture.
PG  - 756-62
AB  - Fluorescence in situ hybridization (FISH) was applied with total genomic DNA 
      extracted from Dasypyrum villosum (L.) Candargy as a probe to characterize 
      chromosome translocations arising from tissue culture in hybrids of Triticum 
      aestivum x (T. durum - D. villosum, amphiploid). Chromosome translocations 
      between wheat and D. villosum occurred in callus cells at an average frequency of 
      1.9%. Translocations existed not only in callus cells but also in regenerants. 
      Three plants with translocation chromosomes were characterized among 66 
      regenerants of T. aestivum 'Chinese Spring' x 'TH1W' and 'NPFP' x 'TH1'. One of 
      them proved to be a reciprocal translocation with an exchange of about one third 
      of a wheat chromosome arm with about one half of a chromosome arm of D. villosum. 
      The breakpoints of the other two translocations were located at, or near 
      centromeres. The results are similar for both callus cells and regenerants and 
      provide further evidence that translocations take place in tissue culture. Other 
      structural chromosomal changes, for example, fragments, telocentrics, 
      dicentromeres, and deletions, as well as numerical alterations including 
      aneuploidy and polyploidy were recorded both in callus cells and regenerants.
FAU - Li, H J
AU  - Li HJ
AD  - Institute of Botany, The Chinese Academy of Sciences, Beijing, People's Republic 
      of China.
FAU - Guo, B H
AU  - Guo BH
FAU - Li, Y W
AU  - Li YW
FAU - Du, L Q
AU  - Du LQ
FAU - Jia, X
AU  - Jia X
FAU - Chu, C C
AU  - Chu CC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Intergenic)
SB  - IM
MH  - Animals
MH  - Chimera
MH  - Chromosome Aberrations
MH  - Culture Techniques/methods
MH  - DNA, Intergenic
MH  - In Situ Hybridization/methods
MH  - *Translocation, Genetic
MH  - Triticum/cytology/*genetics
EDAT- 2000/11/18 11:00
MHDA- 2001/03/07 10:01
CRDT- 2000/11/18 11:00
PHST- 2000/11/18 11:00 [pubmed]
PHST- 2001/03/07 10:01 [medline]
PHST- 2000/11/18 11:00 [entrez]
AID - 10.1139/g00-020 [doi]
PST - ppublish
SO  - Genome. 2000 Oct;43(5):756-62. doi: 10.1139/g00-020.