PMID- 11081984 OWN - NLM STAT- MEDLINE DCOM- 20010301 LR - 20161020 IS - 0831-2796 (Print) IS - 0831-2796 (Linking) VI - 43 IP - 5 DP - 2000 Oct TI - Comparative physical mapping of the 18S-5.8S-26S rDNA in three sorghum species. PG - 918-22 AB - The physical locations of the 18S-5.8S-26S rDNA sequences were examined in three sorghum species by fluorescence in situ hybridization (FISH) using biotin-labeled heterologous 18S-5.8S-26S rDNA probe (pTa71). Each 18S-5.8S-26S rDNA locus occurred at two sites on the chromosomes in Sorghum bicolor (2n = 20) and S. versicolor (2n = 10), but at four sites on the chromosomes of S. halepense (2n = 40) and the tetraploid S. versicolor (2n = 20). Positions of the rDNA loci varied from the interstitial to terminal position among the four accessions of the three sorghum species. The rDNA data are useful for investigation of chromosome evolution and phylogeny. This study excluded S. versicolor as the possible progenitor of S. bicolor. FAU - Sang, Y AU - Sang Y AD - Department of Agronomy, Kansas State University, Manhattan 66506-5501, USA. FAU - Liang, G H AU - Liang GH LA - eng PT - Comparative Study PT - Journal Article PL - Canada TA - Genome JT - Genome JID - 8704544 RN - 0 (DNA, Ribosomal) SB - IM MH - *DNA, Ribosomal MH - Edible Grain/*genetics MH - In Situ Hybridization, Fluorescence MH - Mitosis MH - Physical Chromosome Mapping/*methods MH - Ploidies EDAT- 2000/11/18 11:00 MHDA- 2001/03/07 10:01 CRDT- 2000/11/18 11:00 PHST- 2000/11/18 11:00 [pubmed] PHST- 2001/03/07 10:01 [medline] PHST- 2000/11/18 11:00 [entrez] PST - ppublish SO - Genome. 2000 Oct;43(5):918-22.