PMID- 11082487 OWN - NLM STAT- MEDLINE DCOM- 20010201 LR - 20190614 IS - 0006-8993 (Print) IS - 0006-8993 (Linking) VI - 884 IP - 1--2 DP - 2000 Nov 24 TI - Neuroprotective effects of brain-derived neurotrophic factor in eyes with NMDA-induced neuronal death. PG - 59-67 AB - PURPOSE: To determine if brain-derived neurotrophic factor (BDNF) has a neuroprotective effect against N-methyl-D-aspartate (NMDA)-induced cell death in retina. METHODS: NMDA was injected into the vitreous of rat eyes. NMDA-induced neuronal death was measured by morphometric analyses on cell counts of ganglion cell layer cells and thickness of retinal layers. Also, we conducted additional experiment using retrograde labeling with a fluorescent tracer (Fluoro-Gold) for exact counting of retinal ganglion cells (RGCs). In addition, intravitreal glutamate levels were measured with the use of a high-performance liquid chromatography (HPLC) system. RESULTS: Morphometric analysis of retinal damage in NMDA-injected eyes showed that BDNF could protect inner retinal cells from glutamate receptor-mediated neuronal death. Also, counts of RGCs labeled with a fluorescent tracer showed that BDNF could protect RGCs from glutamate receptor-mediated neuronal death. Furthermore, measurements of intravitreal glutamate levels indicated an increase in this excitatory amino acid in the vitreous after NMDA injection. CONCLUSIONS: Exogenous BDNF can protect inner retinal cells (possible RGCs and amacrine cells) from NMDA-induced neuronal death. However, increased intravitreal glutamate levels in response to NMDA-mediated neurotoxicity may augment retinal degeneration. FAU - Kido, N AU - Kido N AD - Department of Ophthalmology and Visual Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan. FAU - Tanihara, H AU - Tanihara H FAU - Honjo, M AU - Honjo M FAU - Inatani, M AU - Inatani M FAU - Tatsuno, T AU - Tatsuno T FAU - Nakayama, C AU - Nakayama C FAU - Honda, Y AU - Honda Y LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Brain Res JT - Brain research JID - 0045503 RN - 0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Fluorescent Dyes) RN - 0 (Neuroprotective Agents) RN - 0 (Neurotoxins) RN - 0 (Receptors, N-Methyl-D-Aspartate) RN - 0 (Stilbamidines) RN - 3KX376GY7L (Glutamic Acid) RN - 6384-92-5 (N-Methylaspartate) SB - IM MH - Animals MH - Brain-Derived Neurotrophic Factor/metabolism/*pharmacology MH - Cell Count/statistics & numerical data MH - Cell Death/*drug effects/physiology MH - Fluorescent Dyes MH - Glutamic Acid/metabolism MH - Male MH - N-Methylaspartate/*toxicity MH - Nerve Degeneration/chemically induced/*drug therapy/pathology MH - Neurons/*drug effects/metabolism/pathology MH - Neuroprotective Agents/*pharmacology MH - Neurotoxins/metabolism/pharmacology MH - Rats MH - Rats, Sprague-Dawley MH - Receptors, N-Methyl-D-Aspartate/drug effects/metabolism MH - Retina/*drug effects/pathology/physiopathology MH - Retinal Ganglion Cells/drug effects/metabolism/pathology MH - *Stilbamidines MH - Vitreous Body/metabolism EDAT- 2000/11/18 11:00 MHDA- 2001/02/28 10:01 CRDT- 2000/11/18 11:00 PHST- 2000/11/18 11:00 [pubmed] PHST- 2001/02/28 10:01 [medline] PHST- 2000/11/18 11:00 [entrez] AID - S0006899300028870 [pii] AID - 10.1016/s0006-8993(00)02887-0 [doi] PST - ppublish SO - Brain Res. 2000 Nov 24;884(1--2):59-67. doi: 10.1016/s0006-8993(00)02887-0.