PMID- 11083926
OWN - NLM
STAT- MEDLINE
DCOM- 20010222
LR  - 20091119
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 16
IP  - 5
DP  - 2000 Nov
TI  - Cloning and characterization of muscle-specific kinase in chicken.
PG  - 661-73
AB  - Muscle-specific kinase (MuSK) is part of the receptor complex that is involved in 
      the agrin-induced formation of the neuromuscular junction. In the rodent, 
      prominent mRNA expression of MuSK is restricted to skeletal muscle while the 
      expression of agrin can also be detected in brain and certain nonneuronal 
      tissues. The recent identification of Xenopus MuSK reveals that MuSK can be 
      detected in tissues other than skeletal muscle, such as the neural tube, eye 
      vesicles, and spleen. In this study, we describe the cloning and characterization 
      of the chicken ortholog of MuSK and demonstrate that the regulation of MuSK 
      expression in muscle is conserved from avian to rodent. Abundant mRNA expression 
      of MuSK can be detected in early embryonic chick muscle and is up-regulated after 
      nerve injury. More importantly, we also demonstrate that, in the chicken, MuSK 
      mRNA is expressed during development in brain and liver, suggesting possible 
      novel functions for MuSK. Furthermore, the regulatory profile of MuSK expression 
      in chick muscle closely parallels that observed for acetylcholine receptor, in 
      terms of both mRNA expression and protein localization. Finally, studies with 
      paralyzed chicken muscle as well as with cultured chick myotubes demonstrate the 
      dependence of MuSK on both electrical activity and trophic factors.
CI  - Copyright 2000 Academic Press.
FAU - Ip, F C
AU  - Ip FC
AD  - Department of Biochemistry and Biotechnology Research Institute, Hong Kong 
      University of Science and Technology, Clear Water Bay, Hong Kong, China.
FAU - Glass, D G
AU  - Glass DG
FAU - Gies, D R
AU  - Gies DR
FAU - Cheung, J
AU  - Cheung J
FAU - Lai, K O
AU  - Lai KO
FAU - Fu, A K
AU  - Fu AK
FAU - Yancopoulos, G D
AU  - Yancopoulos GD
FAU - Ip, N Y
AU  - Ip NY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Receptors, Cholinergic)
RN  - 4368-28-9 (Tetrodotoxin)
RN  - EC 2.7.10.1 (MUSK protein, human)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Chickens/*genetics
MH  - Cloning, Molecular
MH  - *Gene Expression Regulation, Developmental
MH  - *Gene Expression Regulation, Enzymologic
MH  - Molecular Sequence Data
MH  - Muscle Denervation
MH  - Muscle Fibers, Skeletal/cytology/enzymology
MH  - Muscle, Skeletal/cytology/enzymology/innervation
MH  - Nerve Crush
MH  - Paralysis/chemically induced
MH  - Protein Structure, Tertiary
MH  - Protein-Tyrosine Kinases/chemistry/genetics
MH  - Rats
MH  - Receptor Protein-Tyrosine Kinases/chemistry/*genetics
MH  - *Receptors, Cholinergic
MH  - Sciatic Nerve/physiology
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Tetrodotoxin/pharmacology
MH  - Transcription, Genetic/physiology
EDAT- 2000/11/21 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/11/21 11:00
PHST- 2000/11/21 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/11/21 11:00 [entrez]
AID - S1044-7431(00)90892-3 [pii]
AID - 10.1006/mcne.2000.0892 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 2000 Nov;16(5):661-73. doi: 10.1006/mcne.2000.0892.