PMID- 11084678
OWN - NLM
STAT- MEDLINE
DCOM- 20010111
LR  - 20061115
IS  - 1043-0342 (Print)
IS  - 1043-0342 (Linking)
VI  - 11
IP  - 16
DP  - 2000 Nov 1
TI  - Efficient expression of the tumor-associated antigen MAGE-3 in human dendritic 
      cells, using an avian influenza virus vector.
PG  - 2207-18
AB  - Dendritic cells (DCs) are the most potent inducers of immune reactions. 
      Genetically modified DCs, which express tumor-associated antigens (TAA), can 
      efficiently induce antitumor immunity and thus have a high potential as tools in 
      cancer therapy. The gene delivery is most efficiently achieved by viral vectors. 
      Here, we explored the capacity of influenza virus vectors to transduce TAA genes. 
      These viruses abortively infect DCs without interfering with their 
      antigen-presenting capacity. In contrast to other viruses used for DC 
      transduction, influenza viruses can be efficiently controlled by antiviral 
      pharmaceuticals, lack the ability to integrate into host chromosomes, and fail to 
      establish persistent infections. Genes encoding a melanoma-derived TAA (MAGE-3), 
      or the green fluorescence protein (GFP), were introduced into a high-expression 
      avian influenza virus vector. Monocyte-derived mature DCs infected by these 
      recombinants efficiently produced GFP or MAGE-3. More than 90% of the infected 
      DCs can express a transduced gene. Importantly, these transduced DCs retained 
      their characteristic phenotype and their potent allogeneic T cell stimulatory 
      capacity, and were able to stimulate MAGE-3-specific CD8(+) cytotoxic T cells. 
      Thus influenza virus vectors provide a highly efficient gene delivery system in 
      order to transduce human DCs with TAA, which consequently stimulate TAA-specific 
      T cells.
FAU - Strobel, I
AU  - Strobel I
AD  - Department of Dermatology, University of Erlangen-Nurnberg, D-91052 Erlangen, 
      Germany.
FAU - Krumbholz, M
AU  - Krumbholz M
FAU - Menke, A
AU  - Menke A
FAU - Hoffmann, E
AU  - Hoffmann E
FAU - Dunbar, P R
AU  - Dunbar PR
FAU - Bender, A
AU  - Bender A
FAU - Hobom, G
AU  - Hobom G
FAU - Steinkasserer, A
AU  - Steinkasserer A
FAU - Schuler, G
AU  - Schuler G
FAU - Grassmann, R
AU  - Grassmann R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Hum Gene Ther
JT  - Human gene therapy
JID - 9008950
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Luminescent Proteins)
RN  - 0 (MAGEA3 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Neoplasm/*genetics/metabolism
MH  - CD8-Positive T-Lymphocytes/metabolism
MH  - Cell Line
MH  - Cell Separation
MH  - Dendritic Cells/*metabolism
MH  - Dogs
MH  - Flow Cytometry
MH  - *Gene Transfer Techniques
MH  - Genetic Vectors
MH  - Green Fluorescent Proteins
MH  - Humans
MH  - Immunoblotting
MH  - Immunophenotyping
MH  - Influenza A virus/*genetics
MH  - Luminescent Proteins/genetics/metabolism
MH  - Microscopy, Phase-Contrast
MH  - Neoplasm Proteins/*genetics/*metabolism
MH  - Phenotype
MH  - Plasmids/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocytes, Cytotoxic/metabolism
MH  - Transduction, Genetic
MH  - Tumor Cells, Cultured
EDAT- 2000/11/21 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/21 11:00
PHST- 2000/11/21 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/21 11:00 [entrez]
AID - 10.1089/104303400750035735 [doi]
PST - ppublish
SO  - Hum Gene Ther. 2000 Nov 1;11(16):2207-18. doi: 10.1089/104303400750035735.