PMID- 11085950
OWN - NLM
STAT- MEDLINE
DCOM- 20010118
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 352 Pt 2
IP  - Pt 2
DP  - 2000 Dec 1
TI  - Prosaposin: promoter analysis and central-nervous-system-preferential elements 
      for expression in vivo.
PG  - 549-56
AB  - The expression of prosaposin is temporally and spatially regulated at the 
      transcriptional and post-translational levels. In vitro, the mouse prosaposin 
      promoter contains functional RORE [retinoic acid-receptor-related orphan receptor 
      alpha subunit (RORalpha)-binding element], Sp1 and U (unknown) sites within 310 
      bp directly 5' to the transcription start site and additional elements within 
      2400 bp 5' to the transcription start site. To elucidate promoter regions 
      important to tissue-preferential expression in vivo, transgenic mice were created 
      with 5'-flanking deletions of the prosaposin gene fused to a luciferase reporter. 
      Nearly exclusive expression was observed in cerebrum, cerebellum and eyes of 
      adult transgenic mice containing constructs with 234-310 bp of 5'-flanking DNA. 
      This central nervous system (CNS) expression was due to the presence of RORE and 
      overlapping Sp1 sites in this region. Internal deletion of RORE and the Sp1 
      cluster from the longer constructs with 2400 bp of 5'-flanking DNA significantly 
      diminished expression in the CNS. The appearance of substantial visceral tissue 
      (e.g. liver, spleen, lung, kidney, thymus and heart) expression was obtained with 
      transgenic mice bearing constructs with 742-2400 bp of 5'-flanking DNA. The 
      cellular localization of luciferase reporter-gene expression from these 
      constructs corresponded closely with that for prosaposin. These results define 
      important CNS and visceral regulatory regions in the promoter in vivo and may be 
      sufficient to account for the majority of prosaposin's tissue-preferential 
      expression.
FAU - Sun, Y
AU  - Sun Y
AD  - Division and Program in Human Genetics, Children's Hospital Medical Center, 3333 
      Burnet Avenue, TCHRF 1042, Cincinnati, OH 45229-3039, USA.
FAU - Jin, P
AU  - Jin P
FAU - Witte, D P
AU  - Witte DP
FAU - Grabowski, G A
AU  - Grabowski GA
LA  - eng
GR  - NS 34071/NS/NINDS NIH HHS/United States
GR  - NS 36681/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Glycoproteins)
RN  - 0 (Psap protein, mouse)
RN  - 0 (Saposins)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Animals
MH  - Central Nervous System/*metabolism
MH  - *Gene Expression
MH  - Glycoproteins/*genetics
MH  - Luciferases/genetics
MH  - Mice
MH  - Mice, Transgenic
MH  - *Promoter Regions, Genetic
MH  - Saposins
MH  - Transgenes
PMC - PMC1221488
EDAT- 2000/11/22 11:00
MHDA- 2001/02/28 10:01
PMCR- 2001/06/01
CRDT- 2000/11/22 11:00
PHST- 2000/11/22 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/22 11:00 [entrez]
PHST- 2001/06/01 00:00 [pmc-release]
PST - ppublish
SO  - Biochem J. 2000 Dec 1;352 Pt 2(Pt 2):549-56.