PMID- 11086130
OWN - NLM
STAT- MEDLINE
DCOM- 20010111
LR  - 20200213
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 81
IP  - Pt 12
DP  - 2000 Dec
TI  - Analysis of cyclins in trigeminal ganglia of calves infected with bovine 
      herpesvirus-1.
PG  - 2993-2998
LID - 10.1099/0022-1317-81-12-2993 [doi]
AB  - Following acute infection of cattle with bovine herpesvirus-1 (BHV-1), cyclin 
      expression was examined in trigeminal ganglia (TG). Cyclin A was primarily 
      detected in the nucleus of TG neurons. In contrast, cyclin D1 and cyclin E were 
      detected in the nucleus and cytoplasm of TG neurons. Uninfected or latently 
      infected calves did not express detectable levels of these cyclins in TG neurons. 
      Following dexamethasone-induced reactivation, cyclins D1, E and A were also 
      detected in TG neurons. In situ hybridization of consecutive sections 
      demonstrated that many neurons expressing cyclins contained viral nucleic acid, 
      demonstrating that they were infected. Based on these observations, we 
      hypothesize that BHV-1 infection activates neuronal cyclin expression to enhance 
      productive infection. It is also possible that the stress of neuronal infection 
      or reactivation leads to cyclin expression.
FAU - Winkler, Maria Teresa
AU  - Winkler MT
AD  - Department of Veterinary and Biomedical Sciences, Center for Biotechnology, 
      University of Nebraska, Fair Street at East Campus Loop, Lincoln, NE 68583-0905, 
      USA1.
FAU - Schang, Luis S
AU  - Schang LS
AD  - Department of Veterinary and Biomedical Sciences, Center for Biotechnology, 
      University of Nebraska, Fair Street at East Campus Loop, Lincoln, NE 68583-0905, 
      USA1.
FAU - Doster, Alan
AU  - Doster A
AD  - Department of Veterinary and Biomedical Sciences, Center for Biotechnology, 
      University of Nebraska, Fair Street at East Campus Loop, Lincoln, NE 68583-0905, 
      USA1.
FAU - Holt, Todd
AU  - Holt T
AD  - Department of Veterinary and Biomedical Sciences, Center for Biotechnology, 
      University of Nebraska, Fair Street at East Campus Loop, Lincoln, NE 68583-0905, 
      USA1.
FAU - Jones, Clinton
AU  - Jones C
AD  - Department of Veterinary and Biomedical Sciences, Center for Biotechnology, 
      University of Nebraska, Fair Street at East Campus Loop, Lincoln, NE 68583-0905, 
      USA1.
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Cyclin A)
RN  - 0 (Cyclin E)
RN  - 0 (Cyclins)
RN  - 136601-57-5 (Cyclin D1)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cattle
MH  - Cell Nucleus/metabolism
MH  - Cyclin A/metabolism
MH  - Cyclin D1/metabolism
MH  - Cyclin E/metabolism
MH  - Cyclins/*metabolism
MH  - Cytoplasm/metabolism
MH  - Dexamethasone/pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Herpesviridae Infections/*metabolism/virology
MH  - Herpesvirus 1, Bovine/drug effects/*physiology
MH  - Immunohistochemistry
MH  - Neurons/cytology/metabolism/pathology/virology
MH  - Trigeminal Ganglion/cytology/*metabolism/pathology/*virology
MH  - Virus Activation/drug effects
MH  - Virus Latency/drug effects
EDAT- 2000/11/22 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/22 11:00
PHST- 2000/11/22 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/22 11:00 [entrez]
AID - 10.1099/0022-1317-81-12-2993 [doi]
PST - ppublish
SO  - J Gen Virol. 2000 Dec;81(Pt 12):2993-2998. doi: 10.1099/0022-1317-81-12-2993.