PMID- 11089895
OWN - NLM
STAT- MEDLINE
DCOM- 20010301
LR  - 20191104
IS  - 0940-2993 (Print)
IS  - 0940-2993 (Linking)
VI  - 52
IP  - 5
DP  - 2000 Oct
TI  - Histological observations of palatal malformations in rat embryos induced by 
      retinoic acid treatment.
PG  - 437-44
AB  - Malformations of the palate were induced in white rat embryos following maternal 
      exposure to retinoic acid (tretinoin). Five experimental groups and the controls 
      were treated by the following protocol: Group 1: pregnant rats received 100 mg 
      retinoic acid (RA)/kg b.w. suspended in corn oil on gestational day (GD) 11.5; 
      Group 2: 20 mg RA/kg b.w. from GD 8-12; Group 3: 20 mg RA/kg b.w. from GD 
      7.5-11.5; Group 4: 100 mg RA/kg b.w. on GD 10-11; Group 5: 100 mg RA/kg b.w. on 
      GD 10 and 12; Group 6 received corn oil vehicle from GD 7-14.5; and Group 6: 
      served as non-injected controls. In all retinoic acid treated groups, varying 
      degrees of clefts with occasional attempts of fusion were noted. The severity and 
      frequency of the malformations were dependent on dosage or gestational day of 
      drug treatment. Our results indicate that RA, even at the lowest dose tested (20 
      mg/kg b.w.) severely affects the various tissues constituting the embryonic 
      palatal shelves by altering cell interaction and possibly programmed cell death. 
      These events would then result in lack of or inadequate differentiation with 
      subsequent formation of aberrant craniofacial architecture.
FAU - Emmanouil-Nikoloussi, E N
AU  - Emmanouil-Nikoloussi EN
AD  - Laboratory of Histology-Embryology, Faculty of Medicine, Aristotle University of 
      Thessaloniki, Greece.
FAU - Goret-Nicaise, M
AU  - Goret-Nicaise M
FAU - Foroglou, P
AU  - Foroglou P
FAU - Kerameos-Foroglou, C
AU  - Kerameos-Foroglou C
FAU - Persaud, T V
AU  - Persaud TV
FAU - Thliveris, J A
AU  - Thliveris JA
FAU - Dhem, A
AU  - Dhem A
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Exp Toxicol Pathol
JT  - Experimental and toxicologic pathology : official journal of the Gesellschaft fur 
      Toxikologische Pathologie
JID - 9208920
RN  - 5688UTC01R (Tretinoin)
SB  - IM
MH  - Abnormalities, Drug-Induced/*pathology
MH  - Animals
MH  - Cleft Palate/chemically induced/pathology
MH  - Female
MH  - Palate/*abnormalities/embryology
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Tretinoin/*toxicity
EDAT- 2000/11/23 11:00
MHDA- 2001/03/07 10:01
CRDT- 2000/11/23 11:00
PHST- 2000/11/23 11:00 [pubmed]
PHST- 2001/03/07 10:01 [medline]
PHST- 2000/11/23 11:00 [entrez]
AID - 10.1016/s0940-2993(00)80079-2 [doi]
PST - ppublish
SO  - Exp Toxicol Pathol. 2000 Oct;52(5):437-44. doi: 10.1016/s0940-2993(00)80079-2.