PMID- 11090091
OWN - NLM
STAT- MEDLINE
DCOM- 20010201
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 96
IP  - 12
DP  - 2000 Dec 1
TI  - Polyclonal hematopoiesis with variable telomere shortening in human long-term 
      allogeneic marrow graft recipients.
PG  - 3991-4
AB  - Donor-derived hematopoiesis was assessed in 17 patients who received allogeneic 
      marrow grafts from HLA-matched siblings between 1971 and 1980. Complete blood 
      counts were normal or near normal in all patients except one. Chimerism analyses, 
      using either dual-color XY-chromosome fluorescence in situ hybridization (FISH) 
      or analysis of variable number tandem repeat loci, indicated that 15 out of 16 
      patients had greater than 97% donor-derived hematopoiesis, whereas 1 patient had 
      indeterminate chimerism. All 12 recipients of grafts from female donors exhibited 
      polyclonal hematopoiesis by X-linked clonal analysis with the use of molecular 
      probes. Of the 17 recipients, 9 exhibited a less than 1.0-kilobase shortening of 
      granulocyte telomere length compared with their respective donors, according to 
      terminal restriction fragment analysis or flow-FISH with a fluorescein-labeled 
      peptide nucleic acid probe. These data suggest that under standard 
      transplantation conditions, the stem cell proliferative potential is not 
      compromised during hematopoietic reconstitution. (Blood. 2000;96:3991-3994)
FAU - Mathioudakis, G
AU  - Mathioudakis G
AD  - Fred Hutchinson Cancer Research Center, Seattle, WA 98109-1024, USA. 
      gmathiou@fhcrc.org
FAU - Storb, R
AU  - Storb R
FAU - McSweeney, P A
AU  - McSweeney PA
FAU - Torok-Storb, B
AU  - Torok-Storb B
FAU - Lansdorp, P M
AU  - Lansdorp PM
FAU - Brummendorf, T H
AU  - Brummendorf TH
FAU - Gass, M J
AU  - Gass MJ
FAU - Bryant, E M
AU  - Bryant EM
FAU - Storek, J
AU  - Storek J
FAU - Flowers, M E
AU  - Flowers ME
FAU - Gooley, T
AU  - Gooley T
FAU - Nash, R A
AU  - Nash RA
LA  - eng
GR  - CA15704/CA/NCI NIH HHS/United States
GR  - CA18221/CA/NCI NIH HHS/United States
GR  - HL36444/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
SB  - IM
CIN - Blood. 2001 Aug 1;98(3):888-90. PMID: 11468140
MH  - Adolescent
MH  - Adult
MH  - *Bone Marrow Transplantation/standards
MH  - Cell Division/physiology
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Follow-Up Studies
MH  - Granulocytes/ultrastructure
MH  - Hematopoiesis/*physiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Nuclear Family
MH  - Polymorphism, Restriction Fragment Length
MH  - Sex Factors
MH  - Telomere/*ultrastructure
MH  - Transplantation Chimera
MH  - Transplantation, Homologous
MH  - X Chromosome/ultrastructure
EDAT- 2000/11/23 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/23 11:00
PHST- 2000/11/23 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/23 11:00 [entrez]
AID - S0006-4971(20)48249-7 [pii]
PST - ppublish
SO  - Blood. 2000 Dec 1;96(12):3991-4.