PMID- 11090142
OWN - NLM
STAT- MEDLINE
DCOM- 20010111
LR  - 20190508
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 74
IP  - 24
DP  - 2000 Dec
TI  - Analysis of individual human trigeminal ganglia for latent herpes simplex virus 
      type 1 and varicella-zoster virus nucleic acids using real-time PCR.
PG  - 11464-71
AB  - Herpes simplex virus type 1 (HSV-1) and varicella-zoster virus (VZV) establish 
      latent infections in the peripheral nervous system following primary infection. 
      During latency both virus genomes exhibit limited transcription, with the HSV-1 
      LATs and at least four VZV transcripts consistently detected in latently infected 
      human ganglia. In this study we used real-time PCR quantitation to determine the 
      viral DNA copy number in individual trigeminal ganglia (TG) from 17 subjects. The 
      number of HSV-1 genomes was not significantly different between the left and 
      right TG from the same individual and varied per subject from 42.9 to 677.9 
      copies per 100 ng of DNA. The number of VZV genomes was also not significantly 
      different between left and right TG from the same individual and varied per 
      subject from 37.0 to 3,560.5 copies per 100 ng of DNA. HSV-1 LAT transcripts were 
      consistently detected in ganglia containing latent HSV-1 and varied in relative 
      expression by >500-fold. Of the three VZV transcripts analyzed, only transcripts 
      mapping to gene 63 were consistently detected in latently infected ganglia and 
      varied in relative expression by >2,000-fold. Thus, it appears that, similar to 
      LAT transcription in HSV-1 latently infected ganglia, VZV gene 63 transcription 
      is a hallmark of VZV latency.
FAU - Cohrs, R J
AU  - Cohrs RJ
AD  - Departments of Neurology, University of Colorado Health Sciences Center, Denver, 
      Colorado 80262, USA.
FAU - Randall, J
AU  - Randall J
FAU - Smith, J
AU  - Smith J
FAU - Gilden, D H
AU  - Gilden DH
FAU - Dabrowski, C
AU  - Dabrowski C
FAU - van Der Keyl, H
AU  - van Der Keyl H
FAU - Tal-Singer, R
AU  - Tal-Singer R
LA  - eng
GR  - NS32623/NS/NINDS NIH HHS/United States
GR  - AG06127/AG/NIA NIH HHS/United States
GR  - P01 NS032623/NS/NINDS NIH HHS/United States
GR  - R37 AG006127/AG/NIA NIH HHS/United States
GR  - R01 AG006127/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - DNA, Viral/analysis
MH  - Female
MH  - Genome, Viral
MH  - Herpes Simplex/*virology
MH  - Herpes Zoster/*virology
MH  - Herpesvirus 1, Human/*physiology
MH  - Herpesvirus 3, Human/*physiology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Trigeminal Ganglion/*virology
MH  - *Virus Latency
PMC - PMC112425
EDAT- 2000/11/23 11:00
MHDA- 2001/02/28 10:01
PMCR- 2000/12/01
CRDT- 2000/11/23 11:00
PHST- 2000/11/23 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/23 11:00 [entrez]
PHST- 2000/12/01 00:00 [pmc-release]
AID - 1214 [pii]
AID - 10.1128/jvi.74.24.11464-11471.2000 [doi]
PST - ppublish
SO  - J Virol. 2000 Dec;74(24):11464-71. doi: 10.1128/jvi.74.24.11464-11471.2000.