PMID- 11090189
OWN - NLM
STAT- MEDLINE
DCOM- 20010111
LR  - 20210107
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 74
IP  - 24
DP  - 2000 Dec
TI  - Varicella-zoster virus gene expression in latently infected and explanted human 
      ganglia.
PG  - 11893-8
AB  - A consistent feature of varicella-zoster virus (VZV) latency is the restricted 
      pattern of viral gene expression in human ganglionic tissues. To understand 
      further the significance of this gene restriction, we used in situ hybridization 
      (ISH) to detect the frequency of RNA expression for nine VZV genes in trigeminal 
      ganglia (TG) from 35 human subjects, including 18 who were human immunodeficiency 
      virus (HIV) positive. RNA for VZV gene 21 was detected in 7 of 11 normal and 6 of 
      10 HIV-positive subjects, RNA for gene 29 was detected in 5 of 14 normal and 11 
      of 11 HIV-positive subjects, RNA for gene 62 was detected in 4 of 10 normal and 6 
      of 9 HIV-positive subjects, and RNA for gene 63 was detected in 8 of 17 normal 
      and 12 of 15 HIV-positive subjects. RNA for VZV gene 4 was detected in 2 of 13 
      normal and 4 of 9 HIV-positive subjects, and RNA for gene 18 was detected in 4 of 
      15 normal and 5 of 15 HIV-positive subjects. By contrast, RNAs for VZV genes 28, 
      40, and 61 were rarely or never detected. In addition, immunocytochemical 
      analysis detected the presence of VZV gene 63-encoded protein in five normal and 
      four HIV-positive subjects. VZV RNA was also analyzed in explanted fresh human TG 
      and dorsal root ganglia from five normal human subjects over a period of up to 11 
      days in culture. We found a very different pattern of gene expression in these 
      explants, with transcripts for VZV genes 18, 28, 29, 40, and 63 all frequently 
      detected, presumably as a result of viral reactivation. Taken together, these 
      data provide further support for the notion of significant and restricted viral 
      gene expression in VZV latency.
FAU - Kennedy, P G
AU  - Kennedy PG
AD  - Glasgow University Department of Neurology, Southern General Hospital, Glasgow, 
      United Kingdom. P.G.Kennedy@clinmed.gla.ac.uk
FAU - Grinfeld, E
AU  - Grinfeld E
FAU - Bell, J E
AU  - Bell JE
LA  - eng
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Adult
MH  - Female
MH  - Ganglia/*virology
MH  - *Gene Expression Regulation, Viral
MH  - Herpes Zoster/*genetics/*virology
MH  - Herpesvirus 3, Human/*genetics/*isolation & purification
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - RNA, Viral/analysis
PMC - PMC112472
EDAT- 2000/11/23 11:00
MHDA- 2001/02/28 10:01
PMCR- 2000/12/01
CRDT- 2000/11/23 11:00
PHST- 2000/11/23 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/23 11:00 [entrez]
PHST- 2000/12/01 00:00 [pmc-release]
AID - 1492 [pii]
AID - 10.1128/jvi.74.24.11893-11898.2000 [doi]
PST - ppublish
SO  - J Virol. 2000 Dec;74(24):11893-8. doi: 10.1128/jvi.74.24.11893-11898.2000.