PMID- 11090240
OWN - NLM
STAT- MEDLINE
DCOM- 20010104
LR  - 20181130
IS  - 0896-8411 (Print)
IS  - 0896-8411 (Linking)
VI  - 15
IP  - 4
DP  - 2000 Dec
TI  - A functional and phenotypic study on immune accessory cells isolated from the 
      thyroids of Wistar and autoimmune-prone BB-DP rats.
PG  - 417-24
AB  - Dendritic cells (DCs) comprise a small population of cells in the normal thyroid. 
      These excellent antigen-presenting cells (APCs) are thought to be involved in the 
      initiation of thyroid autoimmune reactions. However it is not known whether the 
      APCs involved in this process are indeed DCs, or thyrocytes. Our aims were as 
      follows: (1) to isolate DCs from the thyroid of normal Wistar rats and BB-DP rats 
      prior to the development of lymphocytic thyroiditis; (2) to determine the T-cell 
      stimulatory capability of such isolated thyroid DCs and to compare this 
      capability to that of BB-DP thyrocytes and splenic DCs; and (3) to investigate 
      the phenotype of isolated thyroid DCs and to compare it to that of splenic DCs; 
      and (4) to investigate the capability of such thyroid DCs to regulate thyrocyte 
      growth and function, and to compare it to our earlier reports demonstrating such 
      capability with splenic DCs. Leukokcytic cell fractions were isolated from the 
      thyroids of BB-DP and control Wistar rats of 7-20 weeks of age. The isolation 
      steps included gentle enzymatic tissue disruption, the collection of non-plastic 
      adherent cells and density gradient centrifugation of these cells to yield a low 
      and a high density non-adherent fraction. The low density cell (LDC) fraction was 
      composed of 50-75% leukocytes in both strains. These leukocytes were almost 
      exclusively ED1+ monocytes or MHC-class II+ DC. The high density cell (HDC) 
      fractions of both strains were composed of about 70% MHC-class II-negative 
      thyrocytes and 30% ED1+ monocytes. The thyroid LDCs of both strains had an APC 
      capability in syngeneic(syn)-MLR comparable to that of splenic DCs. However, the 
      HDCs were extremely poor in syngeneic T cell stimulation. There was a difference 
      in composition between the Wistar and the BB-DP LDC fractions: The Wistar LDCs 
      were composed of 30-35% ED1+ monocytes and 15-20% typical MHC-II+ DCs, while 
      BB-DP LDC fractions contained more ED1+ monocytes (about 70%), but fewer DCs 
      (5-10%). In comparison to splenic DCs, thyroid DCs had a low CD80 and CD86 
      expression in both strains (i.e., an 'immature' phenotype). The LDCs of both 
      animal strains were shown to decrease both basal and TSH-stimulated thyrocyte 
      proliferation and T(3)release by about half. This report shows that a cell 
      fraction enriched for monocytes and DCs can be isolated from the thyroids of both 
      Wistar and BB-DP rats. The cells in this fraction were as capable as splenic DCs 
      to act as T cell stimulators in syn-MLR. Since the thyroid HDCs (predominantly 
      thyrocytes) were very poor at such T cell stimulation, thyroid monocytes and DCs 
      (and not thyrocytes) are the prime candidates to act as immune accessory cells in 
      the initiation of thyroid autoimmunity in the rat. Wistar thyroid LDCs differed 
      in phenotype from BB-DP LDCs. The latter contained a lower percentage of DCs and 
      a higher percentage of their precursors, the monocytes. Interestingly, a defect 
      in the transition of monocytes to DCs has been described in another animal model 
      of autoimmune thyroiditis/insulitis (the NOD mouse), as well as in thyroiditis 
      and diabetic patients.
CI  - Copyright 2000 Academic Press.
FAU - Simons, P J
AU  - Simons PJ
AD  - Department of Immunology, Erasmus University Rotterdam, Rotterdam, The 
      Netherlands.
FAU - Delemarre, F G
AU  - Delemarre FG
FAU - Drexhage, H A
AU  - Drexhage HA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Autoimmun
JT  - Journal of autoimmunity
JID - 8812164
RN  - 06LU7C9H1V (Triiodothyronine)
SB  - IM
MH  - Animals
MH  - Cell Division
MH  - Coculture Techniques
MH  - Dendritic Cells/*physiology
MH  - Lymphocyte Culture Test, Mixed
MH  - Phenotype
MH  - Rats
MH  - Rats, Inbred BB
MH  - Rats, Wistar
MH  - Thyroid Gland/cytology/*immunology
MH  - Thyroiditis, Autoimmune/*immunology
MH  - Triiodothyronine/metabolism
EDAT- 2000/11/25 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/25 11:00
PHST- 2000/11/25 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/25 11:00 [entrez]
AID - S0896-8411(00)90438-4 [pii]
AID - 10.1006/jaut.2000.0438 [doi]
PST - ppublish
SO  - J Autoimmun. 2000 Dec;15(4):417-24. doi: 10.1006/jaut.2000.0438.