PMID- 11091204
OWN - NLM
STAT- MEDLINE
DCOM- 20001213
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 111
IP  - 1
DP  - 2000 Oct
TI  - Ontogeny of natural killer cells and T cells by analysis of BCR-ABL rearrangement 
      from patients with chronic myelogenous leukaemia.
PG  - 216-22
AB  - Chronic myelogenous leukaemia (CML) is a haematological malignant disorder 
      characterized by the Philadelphia chromosome (Ph) and BCR-ABL gene rearrangement. 
      This abnormal fusion gene can be considered to serve as a marker for the 
      transformed cell clone in CML and is found in all cells arising from the same 
      malignant precursor cell. It has been detected in CML cells of the myeloid, 
      monocytic, erythroid and B-lymphocytic lineages. However, it is still arguable as 
      to whether T lymphocytes or natural killer (NK) cells carry this marker. 
      Answering this question would clarify the ontogenic relationship between NK cells 
      and T cells. We examined 12 CML patients and studied the expression of BCR-ABL 
      rearrangement by fluorescence in situ hybridization (FISH) in both NK cells and T 
      cells sorted by flow cytometry. The purity of T cells was 95.6-99.8% and that of 
      NK cells was 95.3-99.3% after sorting. Neither NK cells nor T cells showed any 
      positive BCR-ABL signal with the exception of one patient who recovered from a 
      lymphoid blastic crisis. We speculate that T cells and NK cells originate from 
      BCR-ABL-negative stem cells.
FAU - Cho, E K
AU  - Cho EK
AD  - Department of Internal Medicine, Gachon Medical School Gil Medical Centre, 
      Inchon, South Korea.
FAU - Heo, D S
AU  - Heo DS
FAU - Seol, J G
AU  - Seol JG
FAU - Seo, E J
AU  - Seo EJ
FAU - Chi, H S
AU  - Chi HS
FAU - Kim, E S
AU  - Kim ES
FAU - Lee, Y Y
AU  - Lee YY
FAU - Kim, B K
AU  - Kim BK
FAU - Kim, N K
AU  - Kim NK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Genetic Markers)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Female
MH  - Flow Cytometry
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Gene Expression
MH  - Gene Rearrangement
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Killer Cells, Natural/metabolism
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics/immunology
MH  - Male
MH  - Middle Aged
MH  - T-Lymphocytes/*metabolism
EDAT- 2000/11/25 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/25 11:00
PHST- 2000/11/25 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/25 11:00 [entrez]
AID - bjh2341 [pii]
AID - 10.1046/j.1365-2141.2000.02341.x [doi]
PST - ppublish
SO  - Br J Haematol. 2000 Oct;111(1):216-22. doi: 10.1046/j.1365-2141.2000.02341.x.