PMID- 11091280
OWN - NLM
STAT- MEDLINE
DCOM- 20001214
LR  - 20190513
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 122
IP  - 2
DP  - 2000 Nov
TI  - Glucocorticoids hamper the ex vivo maturation of lung dendritic cells from their 
      low autofluorescent precursors in the human bronchoalveolar lavage: decreases in 
      allostimulatory capacity and expression of CD80 and CD86.
PG  - 232-40
AB  - Dendritic cells (DCs) were prepared from human bronchoalveolar lavage (BAL) 
      cells. We previously reported that, in particular, the CD1a fraction of the low 
      autofluorescent (LAF) cells contains the precursors for DCs: after overnight 
      culture, 40% of the LAF cells change into functionally and phenotypically 
      prototypic dendritic/veiled cells. There are, as yet, no data on the modulatory 
      effects of glucocorticoids (GC) on the maturation and function of such DCs 
      isolated from the human lung. Functional tests (allogeneic mixed lymphocyte 
      reaction: allo-MLR) were therefore performed with CD1a+ LAF cells at different 
      stimulator-to-T-cell ratios and after preincubation with different dexamethasone 
      (DEX) concentrations. DEX caused suppression of the T-cell stimulatory capacity 
      of CD1a+ LAF cells, which was dose-dependent, and more evident at the higher 
      stimulator-to-T-cell ratios. Here, we also show that CD80 and CD86 are normally 
      expressed at low levels on CD1a+ LAF cell-derived DCs compared to other DC 
      populations. This low-level expression of costimulatory molecules is discussed 
      here in relation to the previously reported low-level expression of CD80 (and 
      CD86) on lung DCs in experimental animals. This appears to play a role in a 
      predominant Th2 cell stimulating potential of DC from the lung environment. DEX 
      exposure of CD1a+ LAF cells prevented the upregulation of even this low-level 
      expression of CD80 and CD86. The veiled/dendritic morphology and the expression 
      of other relevant cell surface markers and adhesion molecules was not affected by 
      DEX exposure. It is concluded that DEX hampers the maturation of CD1a+ LAF cells 
      into active lung DCs.
FAU - Verhoeven, G T
AU  - Verhoeven GT
AD  - Department of Immunology, University Hospital Dijkzigt, EMCR, Rotterdam, The 
      Netherlands. Verhoeven@lond.azr.nl
FAU - Van Haarst, J M
AU  - Van Haarst JM
FAU - De Wit, H J
AU  - De Wit HJ
FAU - Simons, P J
AU  - Simons PJ
FAU - Hoogsteden, H C
AU  - Hoogsteden HC
FAU - Drexhage, H A
AU  - Drexhage HA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD1)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD86 protein, human)
RN  - 0 (Glucocorticoids)
RN  - 0 (Isoantigens)
RN  - 0 (Membrane Glycoproteins)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigens, CD/metabolism
MH  - Antigens, CD1/metabolism
MH  - B7-1 Antigen/metabolism
MH  - B7-2 Antigen
MH  - Bronchoalveolar Lavage Fluid/cytology
MH  - Cell Differentiation/drug effects
MH  - Dendritic Cells/*cytology/*drug effects/immunology
MH  - Dexamethasone/*pharmacology
MH  - Fluorescence
MH  - Glucocorticoids/*pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - Isoantigens
MH  - Lung/*cytology/*drug effects/immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Membrane Glycoproteins/metabolism
MH  - Middle Aged
MH  - Stem Cells/cytology/drug effects/immunology
MH  - T-Lymphocytes/immunology
PMC - PMC1905776
EDAT- 2000/11/25 11:00
MHDA- 2001/02/28 10:01
PMCR- 2001/11/01
CRDT- 2000/11/25 11:00
PHST- 2000/11/25 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/25 11:00 [entrez]
PHST- 2001/11/01 00:00 [pmc-release]
AID - cei1354 [pii]
AID - 10.1046/j.1365-2249.2000.01354.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2000 Nov;122(2):232-40. doi: 10.1046/j.1365-2249.2000.01354.x.