PMID- 11092978
OWN - NLM
STAT- MEDLINE
DCOM- 20010201
LR  - 20190513
IS  - 0910-5050 (Print)
IS  - 1876-4673 (Electronic)
IS  - 0910-5050 (Linking)
VI  - 91
IP  - 11
DP  - 2000 Nov
TI  - Intratumor heterogeneity of centromere numerical abnormality in multiple primary 
      gastric cancers: application of fluorescence in situ hybridization with 
      intermittent microwave irradiation on paraffin-embedded tissue.
PG  - 1134-41
AB  - Our recent success in retrieving distinct fluorescence signals in response to 
      centromere specific probing of paraffin-embedded tissues after intermittent 
      microwave (MW) treatment provided the opportunity to analyze chromosome numbers 
      or centromere abnormality in situ in human tumors in various clinicopathological 
      settings. In this study, centromere numerical abnormality (CNA) was investigated 
      by fluorescence in situ hybridization (FISH) in a case of multiple gastric cancer 
      having intratumor histological heterogeneity. The different profiles as 
      determined using a total of 20 specific probes on 4 multifocal lesions in the 
      stomach confirmed the multi-clonality of these tumors. FISH with probes specific 
      for chromosomes 10, 11, 16 and 18 revealed intratumor heterogeneity of the CNA, 
      which corresponded to the histological heterogeneity. Our report clearly 
      demonstrates, for the first time, intratumor heterogeneity of CNA and its 
      association with the histological picture, and substantiates the applicability of 
      the MW-assisted FISH protocol to paraffin-embedded pathological specimens.
FAU - Kobayashi, K
AU  - Kobayashi K
AD  - First Department of Pathology, Hamamatsu University School of Medicine, 
      Hamamatsu, Shizuoka 431-3192, Japan.
FAU - Kitayama, Y
AU  - Kitayama Y
FAU - Igarashi, H
AU  - Igarashi H
FAU - Yoshino, G
AU  - Yoshino G
FAU - Kobayashi, T
AU  - Kobayashi T
FAU - Kazui, T
AU  - Kazui T
FAU - Sugimura, H
AU  - Sugimura H
LA  - eng
PT  - Case Reports
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Jpn J Cancer Res
JT  - Japanese journal of cancer research : Gann
JID - 8509412
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - *Centromere
MH  - Chromosome Aberrations
MH  - DNA, Neoplasm/genetics
MH  - Epithelial Cells/ultrastructure
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/ultrastructure
MH  - Male
MH  - Microsatellite Repeats/genetics
MH  - *Microwaves
MH  - Middle Aged
MH  - Neoplasms, Multiple Primary/*genetics/pathology
MH  - Paraffin Embedding
MH  - Stomach Neoplasms/*genetics/pathology
PMC - PMC5926281
EDAT- 2000/11/28 11:00
MHDA- 2001/02/28 10:01
PMCR- 2000/11/01
CRDT- 2000/11/28 11:00
PHST- 2000/11/28 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/28 11:00 [entrez]
PHST- 2000/11/01 00:00 [pmc-release]
AID - CAE1134 [pii]
AID - 10.1111/j.1349-7006.2000.tb00896.x [doi]
PST - ppublish
SO  - Jpn J Cancer Res. 2000 Nov;91(11):1134-41. doi: 
      10.1111/j.1349-7006.2000.tb00896.x.