PMID- 11093948
OWN - NLM
STAT- MEDLINE
DCOM- 20010102
LR  - 20181130
IS  - 0193-1857 (Print)
IS  - 0193-1857 (Linking)
VI  - 279
IP  - 6
DP  - 2000 Dec
TI  - GLP-2 stimulates intestinal growth in premature TPN-fed pigs by suppressing 
      proteolysis and apoptosis.
PG  - G1249-56
AB  - We wished to determine whether exogenous glucagon-like peptide (GLP)-2 infusion 
      stimulates intestinal growth in parenterally fed immature pigs. Piglets (106-108 
      days gestation) were given parenteral nutrient infusion (TPN), TPN + human GLP-2 
      (25 nmol. kg(-1). day(-1)), or sow's milk enterally (ENT) for 6 days. Intestinal 
      protein synthesis was then measured in vivo after a bolus dose of 
      [1-(13)C]phenylalanine, and degradation was calculated from the difference 
      between protein accretion and synthesis. Crypt cell proliferation and apoptosis 
      were measured in situ by 5-bromodeoxyuridine (BrdU) and terminal dUTP nick-end 
      labeling (TUNEL), respectively. Intestinal protein and DNA accretion rates and 
      villus heights were similar in GLP-2 and ENT pigs, and both were higher (P < 
      0.05) than in TPN pigs. GLP-2 decreased fractional protein degradation rate, 
      whereas ENT increased fractional protein synthesis rate compared with TPN pigs. 
      Percentage of TUNEL-positive cells in GLP-2 and ENT groups was 48 and 64% lower, 
      respectively, than in TPN group (P < 0.05). However, ENT, but not GLP-2, 
      increased percentage of BrdU-positive crypt cells above that in TPN piglets. We 
      conclude that GLP-2 increases intestinal growth in premature, TPN-fed pigs by 
      decreasing proteolysis and apoptosis, whereas enteral nutrition acts via 
      increased protein synthesis and cell proliferation and decreased apoptosis.
FAU - Burrin, D G
AU  - Burrin DG
AD  - United States Department of Agriculture, Agricultural Research Service, 
      Children's Nutrition Research Center, Department of Pediatrics, Baylor College of 
      Medicine, Houston, Texas 77030, USA.
FAU - Stoll, B
AU  - Stoll B
FAU - Jiang, R
AU  - Jiang R
FAU - Petersen, Y
AU  - Petersen Y
FAU - Elnif, J
AU  - Elnif J
FAU - Buddington, R K
AU  - Buddington RK
FAU - Schmidt, M
AU  - Schmidt M
FAU - Holst, J J
AU  - Holst JJ
FAU - Hartmann, B
AU  - Hartmann B
FAU - Sangild, P T
AU  - Sangild PT
LA  - eng
GR  - R01-HD-33920/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol Gastrointest Liver Physiol
JT  - American journal of physiology. Gastrointestinal and liver physiology
JID - 100901227
RN  - 0 (Dietary Proteins)
RN  - 0 (Glucagon-Like Peptide 2)
RN  - 0 (Peptides)
RN  - 62340-29-8 (Glucagon-Like Peptides)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - *Apoptosis
MH  - Dietary Proteins/*metabolism
MH  - Enteral Nutrition
MH  - Female
MH  - Gastric Mucosa/metabolism
MH  - Glucagon-Like Peptide 2
MH  - Glucagon-Like Peptides
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Intestinal Mucosa/metabolism
MH  - Intestine, Large/drug effects/growth & development/metabolism
MH  - Intestines/drug effects/*growth & development
MH  - Male
MH  - Pancreas/drug effects/growth & development/metabolism
MH  - *Parenteral Nutrition, Total
MH  - Peptides/blood/*pharmacology
MH  - Stomach/drug effects/growth & development
MH  - Swine
EDAT- 2000/11/30 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/30 11:00
PHST- 2000/11/30 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/30 11:00 [entrez]
AID - 10.1152/ajpgi.2000.279.6.G1249 [doi]
PST - ppublish
SO  - Am J Physiol Gastrointest Liver Physiol. 2000 Dec;279(6):G1249-56. doi: 
      10.1152/ajpgi.2000.279.6.G1249.