PMID- 11094869
OWN - NLM
STAT- MEDLINE
DCOM- 20001222
LR  - 20061115
IS  - 1081-5589 (Print)
IS  - 1081-5589 (Linking)
VI  - 48
IP  - 6
DP  - 2000 Nov
TI  - Induction of monocyte chemoattractant protein-1 by Mycoplasma hominis in 
      respiratory epithelial cells.
PG  - 457-64
AB  - BACKGROUND: Very small preterm infants who have genital mycoplasmas isolated from 
      the trachea are at increased risk to develop bronchopulmonary dysplasia (BPD). 
      The early stages of BPD are characterized by inflammation. Recruitment and 
      activation of mononuclear cells in response to mycoplasmas may be important in 
      the early stage of the disease. Lung epithelial cell production of monocyte 
      chemoattractant protein-1 (MCP-1), a protein that attracts and activates 
      mononuclear cells, could be critical in the regulation of mononuclear cell 
      migration to the lung. METHODS: We examined the potential of Mycoplasma hominis 
      (Mh) to induce MCP-1 gene expression and protein production in A549 cells, a 
      pulmonary epithelial cell line with characteristics of type II cells. RESULTS: 
      Live or heat-inactivated Mh induces MCP-1 mRNA and protein in a dose- and 
      time-dependent manner. Stimulation of MCP-1 by Mh was not inhibited by 50 
      micrograms/mL of polymyxin B, interleukin (IL)-1ra, or neutralizing antibodies to 
      IL-1 alpha, IL-1 beta, or tumor necrosis factor alpha (TNF-alpha). IL-1 alpha, 
      IL-1 beta, and TNF-alpha were not detected in conditioned media of Mh-stimulated 
      A549 cells. CONCLUSIONS: These data suggest that Mh may participate in the 
      inflammatory component of BPD by directly inducing epithelial cell production of 
      cytokines that recruit and activate mononuclear cells.
FAU - Baier, J
AU  - Baier J
AD  - Department of Pediatrics, Louisiana State University Medical Center, Shreveport 
      71130-3932, USA. jbaier@lsumc.edu
FAU - Kruger, T E
AU  - Kruger TE
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Investig Med
JT  - Journal of investigative medicine : the official publication of the American 
      Federation for Clinical Research
JID - 9501229
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Bronchopulmonary Dysplasia/etiology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*genetics
MH  - Epithelial Cells/metabolism
MH  - Humans
MH  - Infant, Newborn
MH  - Lung/*metabolism
MH  - Mycoplasma hominis/*physiology
MH  - RNA, Messenger/analysis
EDAT- 2000/11/30 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/30 11:00
PHST- 2000/11/30 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/30 11:00 [entrez]
PST - ppublish
SO  - J Investig Med. 2000 Nov;48(6):457-64.