PMID- 11095294
OWN - NLM
STAT- MEDLINE
DCOM- 20010531
LR  - 20190815
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 23
IP  - 6
DP  - 2000 Nov
TI  - Immunochemical-based assays in the bioanalysis of immunoglobulins.
PG  - 939-46
AB  - Intravenous immunoglobulin (IVIG) preparations consist of IgG derived from a pool 
      of human plasma of healthy individuals and have been used as routine treatment of 
      patients with primary and secondary immunodeficiencies, autoimmune, and/or 
      inflammatory diseases. Emergence of new infectious agents and development of 
      antibiotic resistance in many bacteria have posed serious problems in the 
      treatment of infections. Since IVIGs contain natural antibodies that occur in the 
      healthy population, their administration to immunocompromised hosts either as a 
      prophylactic agent or as complementary treatment to the usual antimicrobial 
      treatment have been studied. Contradictory results obtained by several clinical 
      studies in respect to the clinical efficacy of IVIGs have in part been ascribed 
      to the poor characterization of IVIG preparations in terms of their specific 
      antibody content against the various pathogenic microorganisms. Immunoassays 
      constitute a promising tool for bioanalysis of IVIGs thanks to the high 
      sensitivity, repeatability and ease of implementation. Ensuring high selectivity, 
      enzyme immunoassays have been used for determination of the levels of 
      pathogen-specific antibodies in IVIG preparations. In this review, the 
      application of immunoassays monitoring such specific antibodies in IVIGs and the 
      relationship of estimated titers with their in vitro opsonic activity are 
      summarized. The relationship of the content of specific antibodies in IVIGs and 
      their functional efficacy with the outcome of clinical studies including patients 
      with primary immunodeficiencies and premature neonates treated with IVIGs is also 
      discussed.
FAU - Lamari, F
AU  - Lamari F
AD  - Department of Chemistry, University of Patras, Greece.
FAU - Anastassiou, E D
AU  - Anastassiou ED
FAU - Dimitracopoulos, G
AU  - Dimitracopoulos G
FAU - Karamanos, N K
AU  - Karamanos NK
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Immunoglobulins, Intravenous)
SB  - IM
MH  - *Antibodies, Bacterial/immunology/isolation & purification
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Humans
MH  - *Immunoglobulins, Intravenous/analysis/immunology/therapeutic use
RF  - 33
EDAT- 2000/11/30 11:00
MHDA- 2001/06/02 10:01
CRDT- 2000/11/30 11:00
PHST- 2000/11/30 11:00 [pubmed]
PHST- 2001/06/02 10:01 [medline]
PHST- 2000/11/30 11:00 [entrez]
AID - S0731708500004052 [pii]
AID - 10.1016/s0731-7085(00)00405-2 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2000 Nov;23(6):939-46. doi: 10.1016/s0731-7085(00)00405-2.