PMID- 11095525
OWN - NLM
STAT- MEDLINE
DCOM- 20010531
LR  - 20220227
IS  - 0959-4965 (Print)
IS  - 0959-4965 (Linking)
VI  - 11
IP  - 16
DP  - 2000 Nov 9
TI  - Nitric oxide activation of TrkB through peroxynitrite.
PG  - 3593-7
AB  - NIH-3T3 cells stably transfected with TrkB, the receptor for brain-derived 
      neurotrophic factor (BDNF), were used to study the effects of NO and 
      peroxynitrite on TrkB. 3-Morpholinosydnonimine (SIN-1), a donor of NO and O2- 
      which immediately react to form peroxynitrite, induced TrkB tyrosine 
      phosphorylation in a dose-dependent relationship from 2 to 40 mM. TrkB 
      phosphorylation by SIN-1 was blocked by superoxide dismutase, which converts O2 
      to H2O2 and prevents its reaction with NO to form peroxynitrite, and by K252a, an 
      inhibitor of TrkB phosphorylation by BDNF. Treatment with NO or O2- alone did not 
      activate TrkB. Treatment directly with 1-4 mM peroxynitrite resulted in a 
      dose-dependent increase in tyrosine phosphorylation of TrkB. SIN-1 treatment 
      induced tyrosine phosphorylation of phospholipase C-gamma1 (PLC-gamma1) and 
      induced its binding with activated TrkB, similar to that seen with BDNF 
      downstream signaling pathways. These studies demonstrate activation of TrkB 
      through peroxynitrite.
FAU - Yuen, E C
AU  - Yuen EC
AD  - Department of Neurology, University of Washington, Seattle 98195, USA.
FAU - Gunther, E C
AU  - Gunther EC
FAU - Bothwell, M
AU  - Bothwell M
LA  - eng
GR  - AG00649/AG/NIA NIH HHS/United States
GR  - NS33200/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Carbazoles)
RN  - 0 (Indole Alkaloids)
RN  - 0 (Isoenzymes)
RN  - 0 (Nitrates)
RN  - 0 (Nitric Oxide Donors)
RN  - 0 (Oxidants)
RN  - 0 (Recombinant Proteins)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - 26404-66-0 (peroxynitric acid)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 5O5U71P6VQ (linsidomine)
RN  - 97161-97-2 (staurosporine aglycone)
RN  - D46583G77X (Molsidomine)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.7.10.1 (Receptor, trkB)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacology
MH  - Carbazoles/pharmacology
MH  - Humans
MH  - Indole Alkaloids
MH  - Isoenzymes/metabolism
MH  - Mice
MH  - Molsidomine/analogs & derivatives/pharmacology
MH  - Nitrates/*pharmacology
MH  - Nitric Oxide/*physiology
MH  - Nitric Oxide Donors/pharmacology
MH  - Oxidants/pharmacology
MH  - Phospholipase C gamma
MH  - Phosphorylation
MH  - Phosphotyrosine/metabolism
MH  - Rats
MH  - Receptor, trkB/drug effects/*physiology
MH  - Recombinant Proteins/drug effects/metabolism/pharmacology
MH  - Superoxide Dismutase/pharmacology
MH  - Transfection
MH  - Type C Phospholipases/metabolism
EDAT- 2000/11/30 11:00
MHDA- 2001/06/02 10:01
CRDT- 2000/11/30 11:00
PHST- 2000/11/30 11:00 [pubmed]
PHST- 2001/06/02 10:01 [medline]
PHST- 2000/11/30 11:00 [entrez]
AID - 10.1097/00001756-200011090-00038 [doi]
PST - ppublish
SO  - Neuroreport. 2000 Nov 9;11(16):3593-7. doi: 10.1097/00001756-200011090-00038.