PMID- 11095607
OWN - NLM
STAT- MEDLINE
DCOM- 20001222
LR  - 20061115
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 41
IP  - 13
DP  - 2000 Dec
TI  - Differential expression of matrix metalloproteinases and their tissue inhibitors 
      at the advancing pterygium head.
PG  - 4142-9
AB  - PURPOSE: Pterygia are a proliferative and inflammatory growth of limbal 
      epithelial stem cell origin, characterized by corneal tissue invasion and 
      extensive matrix remodeling including the destruction of Bowman's layer (BL). The 
      purpose of this study was to determine the expression of matrix 
      metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) at the advancing 
      pterygium edge. METHODS: Formalin-fixed, paraffin-embedded whole eyes (n = 11) 
      with pterygia attached, were serially sectioned and analyzed 
      immunohistochemically to determine the spatial distribution of four MMPs and 
      three TIMPs. Tear samples were collected from other patients with pterygia (n = 
      11) and displayed by gelatin zymography. RESULTS: Collagenase-1 was expressed by 
      pterygium epithelial cells, corneal stromal fibroblasts and pterygium fibroblasts 
      that had migrated between the epithelium and BL at the advancing pterygium edge. 
      Collagenase-3 and gelatinases A and B were detected in all pterygia, intensely 
      staining columnar epithelial cells directly adjacent to the denatured BL. In 
      addition, gelatinase A immunoreactivity was observed on BL. Immunoreactivity for 
      TIMP-1 and -3 paralleled that of the gelatinases, with more intense staining in 
      epithelial cells and fibroblasts where BL was absent. TIMP-2 was faintly detected 
      in pterygium epithelial cells but intensely stained pterygium fibroblasts. 
      Gelatinase B was the most abundant gelatinolytic enzyme present in tears, 
      elevated approximately twofold in eyes with pterygia versus the contralateral 
      control eyes. CONCLUSIONS: This investigation is the first to identify the 
      expression pattern of MMPs and TIMPs at the advancing pterygium edge in specimens 
      of human eyes and in tears derived from patients with pterygia. These enzymes may 
      be responsible for the destruction of BL, and their pattern of differential 
      expression suggests that each may play a selective role in the pathogenesis of 
      pterygia.
FAU - Di Girolamo, N
AU  - Di Girolamo N
AD  - Inflammation Research Unit, School of Pathology, University of New South Wales, 
      Australia. nldigirolamo@unsw.edu.au
FAU - Wakefield, D
AU  - Wakefield D
FAU - Coroneo, M T
AU  - Coroneo MT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Eye Proteins)
RN  - 0 (Protease Inhibitors)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 0 (tear proteins)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Eye Proteins/metabolism
MH  - Female
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Male
MH  - Matrix Metalloproteinases/*metabolism
MH  - Middle Aged
MH  - Protease Inhibitors/*metabolism
MH  - Pterygium/*enzymology/pathology
MH  - Tears/metabolism
MH  - Tissue Inhibitor of Metalloproteinases/*metabolism
EDAT- 2000/11/30 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/30 11:00
PHST- 2000/11/30 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/30 11:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 2000 Dec;41(13):4142-9.