PMID- 11095646
OWN - NLM
STAT- MEDLINE
DCOM- 20010208
LR  - 20210513
IS  - 1046-6673 (Print)
IS  - 1046-6673 (Linking)
VI  - 11
IP  - 12
DP  - 2000 Dec
TI  - Expression of the chemokine monocyte chemoattractant protein-1 and its receptor 
      chemokine receptor 2 in human crescentic glomerulonephritis.
PG  - 2231-2242
LID - 10.1681/ASN.V11122231 [doi]
AB  - Crescents are morphologic manifestations of severe glomerular injury. Several 
      chemokines and their receptors have been demonstrated to be involved in animal 
      models of crescentic glomerulonephritis (cGN) and are potential targets for 
      therapeutic interventions. Therefore, the expression of monocyte chemoattractant 
      protein-1 (MCP-1), its receptor chemokine receptor 2B (CCR2B), and CCR5 in human 
      cGN was studied. MCP-1 and CCR2B mRNA expression was evaluated, by in situ 
      hybridization, in serial sections of 23 renal biopsies from patients with cGN. T 
      cells, macrophages, and CCR5-expressing cells were examined by 
      immunohistochemical analysis. MCP-1 mRNA was expressed by cells in crescents, 
      parietal epithelium, and tubular epithelium, as well as by infiltrating 
      leukocytes in the tubulointerstitium. The expression of CCR2B mRNA was observed 
      in cells in glomeruli and crescents and in infiltrating leukocytes in the 
      tubulointerstitium. CCR2B mRNA expression could not be clearly localized to 
      intrinsic renal cells; evidence that most of the CCR2B-expressing cells were 
      leukocytes is provided. CD3-positive T cells formed the major part of the 
      interstitial cell infiltrates but were rare within the glomerular tufts. 
      CD68-positive macrophages constituted a major population of infiltrating cells in 
      crescents and contributed significantly to the interstitial infiltrates. The 
      number of glomerular macrophages was associated with the number of MCP-1- and 
      CCR2B-positive glomerular cells. Expression of CCR2B was significantly correlated 
      with interstitial CD3-positive T cells. CCR5 expression was restricted to 
      infiltrating leukocytes and was correlated quantitatively and by localization 
      with interstitial CD3-positive T cells and CD68-positive macrophages. These first 
      morphologic data on the distribution of CCR2-positive cells in human cGN suggest 
      differential effects of chemokines and their receptors on the distribution of 
      infiltrating leukocytes in different compartments of the kidney.
FAU - Segerer, Stephan
AU  - Segerer S
AD  - Department of Pathology, University of Washington, Seattle, Washington.
FAU - Cui, Yan
AU  - Cui Y
AD  - Department of Pathology, University of Washington, Seattle, Washington.
FAU - Hudkins, Kelly L
AU  - Hudkins KL
AD  - Department of Pathology, University of Washington, Seattle, Washington.
FAU - Goodpaster, Tracy
AU  - Goodpaster T
AD  - Department of Pathology, University of Washington, Seattle, Washington.
FAU - Eitner, Frank
AU  - Eitner F
AD  - Medizinische Klinik II, Klinikum der Rheinisch-Westfaelischen Technischen 
      Hoshschule, Aachen, Germany.
FAU - Mack, Matthias
AU  - Mack M
AD  - Medizinische Poliklinik, Klinikum der Universitaet, Munich, Germany.
FAU - Schlondorff, Detlef
AU  - Schlondorff D
AD  - Medizinische Poliklinik, Klinikum der Universitaet, Munich, Germany.
FAU - Alpers, Charles E
AU  - Alpers CE
AD  - Department of Pathology, University of Washington, Seattle, Washington.
LA  - eng
GR  - DK47659/DK/NIDDK NIH HHS/United States
GR  - HL63652/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Am Soc Nephrol
JT  - Journal of the American Society of Nephrology : JASN
JID - 9013836
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CCR2 protein, human)
RN  - 0 (CD3 Complex)
RN  - 0 (CD68 antigen, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/metabolism
MH  - CD3 Complex/metabolism
MH  - Cell Count
MH  - Chemokine CCL2/*metabolism
MH  - Glomerulonephritis/*metabolism/*pathology
MH  - Humans
MH  - Kidney Glomerulus/metabolism/pathology
MH  - Kidney Tubules/metabolism/pathology
MH  - Macrophages/pathology
MH  - RNA, Messenger/metabolism
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/genetics/*metabolism
MH  - T-Lymphocytes/pathology
EDAT- 2000/11/30 11:00
MHDA- 2001/03/03 10:01
CRDT- 2000/11/30 11:00
PHST- 2000/11/30 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/11/30 11:00 [entrez]
AID - 11/12/2231 [pii]
AID - 10.1681/ASN.V11122231 [doi]
PST - ppublish
SO  - J Am Soc Nephrol. 2000 Dec;11(12):2231-2242. doi: 10.1681/ASN.V11122231.