PMID- 11096389
OWN - NLM
STAT- MEDLINE
DCOM- 20001219
LR  - 20190906
IS  - 1076-0512 (Print)
IS  - 1076-0512 (Linking)
VI  - 26
IP  - 11
DP  - 2000 Nov
TI  - Increased glycosaminoglycans production in sclerosing basal cell 
      carcinoma-derived fibroblasts and stimulation of normal skin fibroblast 
      glycosaminoglycans production by a cytokine-derived from sclerosing basal cell 
      carcinoma.
PG  - 1029-36
AB  - Sclerosing basal cell carcinoma (S-BCC) is characterized by an abundant stroma. 
      There is evidence that some tumor cells secrete cytokines that are mitogenic for 
      stromal fibroblasts (FBs). From this study we report increased glycosaminoglycan 
      (GAG) production by cultures of S-BCC FBs in comparison to cultures of nodular 
      BCC (N-BCC) FBs and normal skin FBs. GAG production was measured by 
      cetylpyridinium chloride precipitation of incorporated [3H]-glucosamine. The 
      sclerosing BCC FBs demonstrated a significant increase in production of GAG over 
      control FBs (P <.001) and over N-BCC FBs (P<.001). Values reported as a mean 
      percentage +/- SEM for GAG production by S-BCC over control normal skin FBs are 
      359+/-28 and over N-BCC FBs are 266+/-27. In additional experiments, cell extract 
      dilutions from S-BCC tumor, normal dermis, and normal epidermis were incubated 
      with cultures of normal skin FBs. S-BCC-conditioned media was also incubated with 
      normal FBs and GAG production was measured. For both S-BCC extracts and 
      conditioned media, a dose response curve was established showing increased GAG 
      production by normal FBs in relation to increasing the concentration of S-BCC 
      extract or conditioned media. When S-BCC extract was added to normal FBs there 
      was increased GAG production in comparison to normal FBs incubated with dermal or 
      epidermal extracts (P<.001) for both. Two growth factors, transforming growth 
      factor-beta (TGF-beta) and platelet-derived growth factor (PDGF), already known 
      to be mitogenic for FBs, were incubated with N-BCC and normal FBs in an effort to 
      elucidate the potential cytokine(s) released by S-BCC, causing increased GAG 
      production by surrounding FBs. Neither of these cytokines proved to be effective 
      in promoting a significant increase in GAG production. Our findings support the 
      hypothesis that BCCs release factors that alter stromal FB production of GAG.
FAU - Moy, R L
AU  - Moy RL
AD  - UCLA Medical Plaza, Los Angeles, California 90024, USA. rmoy@ucla.edu
FAU - Potter, T S
AU  - Potter TS
FAU - Uitto, J
AU  - Uitto J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Dermatol Surg
JT  - Dermatologic surgery : official publication for American Society for Dermatologic 
      Surgery [et al.]
JID - 9504371
RN  - 0 (Cytokines)
RN  - 0 (Glycosaminoglycans)
SB  - IM
MH  - Carcinoma, Basal Cell/*metabolism/pathology
MH  - Cells, Cultured
MH  - Cytokines/isolation & purification/*pharmacology
MH  - Fibroblasts/*metabolism
MH  - Glycosaminoglycans/*biosynthesis
MH  - Humans
MH  - Sclerosis/metabolism
MH  - Skin/cytology/*metabolism
MH  - Skin Neoplasms/*metabolism/pathology
MH  - Tumor Cells, Cultured
EDAT- 2000/11/30 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/11/30 11:00
PHST- 2000/11/30 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/11/30 11:00 [entrez]
AID - dsu00232 [pii]
AID - 10.1046/j.1524-4725.2000.0260111029.x [doi]
PST - ppublish
SO  - Dermatol Surg. 2000 Nov;26(11):1029-36. doi: 
      10.1046/j.1524-4725.2000.0260111029.x.