PMID- 11097877
OWN - NLM
STAT- MEDLINE
DCOM- 20010215
LR  - 20220310
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 66
IP  - 12
DP  - 2000 Dec
TI  - Community composition of marine bacterioplankton determined by 16S rRNA gene 
      clone libraries and fluorescence in situ hybridization.
PG  - 5116-22
AB  - We determined the compositions of bacterioplankton communities in surface waters 
      of coastal California using clone libraries of 16S rRNA genes and fluorescence in 
      situ hybridization (FISH) in order to compare the community structures inferred 
      from these two culture-independent approaches. The compositions of two clone 
      libraries were quite similar to those of clone libraries of marine 
      bacterioplankton examined by previous studies. Clones from gamma-proteobacteria 
      comprised ca. 28% of the libraries, while approximately 55% of the clones came 
      from alpha-proteobacteria, which dominated the clone libraries. The 
      Cytophaga-Flavobacter group and three others each comprised 10% or fewer of the 
      clone libraries. The community composition determined by FISH differed 
      substantially from the composition implied by the clone libraries. The 
      Cytophaga-Flavobacter group dominated 8 of the 11 communities assayed by FISH, 
      including the two communities assayed using clone libraries. On average only 10% 
      of DAPI (4', 6'-diamidino-2-phenylindole)-stained bacteria were detected by FISH 
      with a probe for alpha-proteobacteria, but 30% of DAPI-stained bacteria appeared 
      to be in the Cytophaga-Flavobacter group as determined by FISH. 
      alpha-Proteobacteria were greatly overrepresented in clone libraries compared to 
      their relative abundance determined by FISH, while the Cytophaga-Flavobacter 
      group was underrepresented in clone libraries. Our data show that the 
      Cytophaga-Flavobacter group can be a numerically dominant component of coastal 
      marine bacterioplankton communities.
FAU - Cottrell, M T
AU  - Cottrell MT
AD  - College of Marine Studies, University of Delaware, Lewes, Delaware 19958, USA.
FAU - Kirchman, D L
AU  - Kirchman DL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (DNA Primers)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Alphaproteobacteria/genetics/isolation & purification
MH  - Animals
MH  - Bacteria/*genetics/isolation & purification
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - Cytophaga/genetics/isolation & purification
MH  - DNA Primers/genetics
MH  - Ecosystem
MH  - Gammaproteobacteria/genetics/isolation & purification
MH  - Gene Library
MH  - Genes, Bacterial
MH  - In Situ Hybridization, Fluorescence
MH  - Plankton/*genetics/isolation & purification
MH  - RNA, Bacterial/*genetics
MH  - RNA, Ribosomal, 16S/*genetics
MH  - *Water Microbiology
PMC - PMC92431
EDAT- 2000/12/01 11:00
MHDA- 2001/03/03 10:01
PMCR- 2000/12/01
CRDT- 2000/12/01 11:00
PHST- 2000/12/01 11:00 [pubmed]
PHST- 2001/03/03 10:01 [medline]
PHST- 2000/12/01 11:00 [entrez]
PHST- 2000/12/01 00:00 [pmc-release]
AID - 0846 [pii]
AID - 10.1128/AEM.66.12.5116-5122.2000 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2000 Dec;66(12):5116-22. doi: 
      10.1128/AEM.66.12.5116-5122.2000.