PMID- 11101274
OWN - NLM
STAT- MEDLINE
DCOM- 20001222
LR  - 20190915
IS  - 1031-3613 (Print)
IS  - 1031-3613 (Linking)
VI  - 11
IP  - 7-8
DP  - 1999
TI  - Matrix metalloproteinases and their endogenous inhibitors during the implantation 
      period in the rat uterus.
PG  - 395-402
AB  - Rats were studied to determine if matrix metalloproteinases (MMPs) and tissue 
      inhibitors of MMPs (TIMPs) were detectable in developing embryonic and uterine 
      tissues during the implantation period; that is, Days 6-8 of pregnancy. Tissue 
      extracts were studied by zymography, reverse zymography and Western blotting. 
      Immunohistochemistry procedures using antibodies against MMPs and TIMPs were 
      applied to tissue sections that passed through implantation sites. The MMP 
      inhibitor, doxycycline, was injected intraluminally into uterine horns of 
      pseudopregnant animals. MMP-2 and -9, and TIMP-2 were detected in gelatin and 
      reverse zymographs, respectively, from extracts of implantation sites on each day 
      studied. Western blots defined bands corresponding to MMP-1, but casein 
      zymographs did not consistently show evidence of extractable MMP-1 or -3. In 
      tissue sections the primary decidual tissue area stained consistently for TIMP-2 
      and on Days 7 and 8 this area also had positive staining for MMP-1 in close 
      proximity to the implanting embryo. On Day 8 positive staining in the outer 
      stromal tissue was detected using an antibody against MMP-9, and another MMP 
      (possibly MMP-3) was localized exclusively in ectoplacental cone/trophoblastic 
      cells. Intraluminal injection of doxycycline did not significantly prevent the 
      growth of the uterine horns following surgical induction of pseudopregnancy on 
      Day 5. The strong localization of TIMP-2 in the primary decidual tissue is 
      similar to that reported previously for TIMP-3, indicating that these inhibitors 
      have a role in decidualization, including the regulation of trophoblastic 
      invasion.
FAU - Hurst, P R
AU  - Hurst PR
AD  - Department of Anatomy and Structural Biology, School of Medical Sciences, 
      University of Otago, Dunedin, New Zealand. peter.hurst@stonebow.otago.ac.nz
FAU - Palmay, R D
AU  - Palmay RD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-3)
RN  - 0 (Tissue Inhibitor of Metalloproteinases)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
RN  - N12000U13O (Doxycycline)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Decidua/enzymology
MH  - Doxycycline/pharmacology
MH  - Electrophoresis
MH  - *Embryo Implantation
MH  - Enzyme Inhibitors/pharmacology
MH  - Female
MH  - Immunohistochemistry
MH  - Matrix Metalloproteinase 1/analysis
MH  - Matrix Metalloproteinase 2/analysis
MH  - Matrix Metalloproteinase 9/analysis
MH  - Matrix Metalloproteinase Inhibitors
MH  - Matrix Metalloproteinases/*analysis
MH  - Pregnancy
MH  - Pseudopregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Time Factors
MH  - Tissue Inhibitor of Metalloproteinase-1/analysis
MH  - Tissue Inhibitor of Metalloproteinase-2/analysis
MH  - Tissue Inhibitor of Metalloproteinase-3/analysis
MH  - Tissue Inhibitor of Metalloproteinases/*analysis
MH  - Uterus/*enzymology
EDAT- 2000/12/02 11:00
MHDA- 2001/02/28 10:01
CRDT- 2000/12/02 11:00
PHST- 2000/12/02 11:00 [pubmed]
PHST- 2001/02/28 10:01 [medline]
PHST- 2000/12/02 11:00 [entrez]
AID - 10.1071/rd99021 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 1999;11(7-8):395-402. doi: 10.1071/rd99021.